Sun Lei, Mao Dongwei, Cai Yan, Tan Wenhua, Hao Yanlan, Li Lin, Liu Wei
Department of Gynaecology and Obstetrics, The Fourth Affiliated Hospital of Harbin Medical University Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Medicine (Baltimore). 2016 Dec;95(52):e5537. doi: 10.1097/MD.0000000000005537.
Preeclampsia (PE) is a common pregnancy-specific disorder associated with significant maternal and fetal morbidity and mortality worldwide.The present study was performed to investigate the role of a CXC chemokine interleukin-8 (IL-8), in the pathogenesis of PE. IL-8 expression levels were assessed in placental and serum samples from 160 pregnant women with PE (N = 68 severe, 92 mild) and 140 healthy donors.Results from enzyme-linked immunosorbent assay showed that the concentration of serum IL-8 in PE patients (180.27 ± 5.81 ng/L) was significantly higher than that in healthy controls (41.57 ± 5.67 ng/L). Patients with severe PE had even higher serum IL-8 levels. Similar messenger RNA and protein expression patterns of IL-8 in placental tissues were confirmed by quantitative real-time polymerase chain reaction and immunohistochemical assay (N = 30 each in the mild PE, severe PE, and control groups). In addition, single nucleotide polymorphisms of IL-8 gene were detected with polymerase chain reaction-restricted fragment length polymorphism/SSP. The frequency of IL-8-251A allele was significantly higher than that in controls (58.4% vs 48.9%, P < 0.05). The occurrence frequency of haplotype -353A/-251A/+678T (AAT) in PE subjects was 27.2% as compared to 21.9% in the control participants (P < 0.05).Our study reveals that IL-8 expression is positively associated with the severity of PE. Presence of haplotype AAT in pregnant women appears to be a risk factor for PE.
子痫前期（PE）是一种常见的妊娠特异性疾病，在全球范围内与孕产妇和胎儿的高发病率及死亡率相关。本研究旨在探讨CXC趋化因子白细胞介素-8（IL-8）在PE发病机制中的作用。对160例PE孕妇（68例重度，92例轻度）和140例健康捐赠者的胎盘及血清样本进行IL-8表达水平评估。酶联免疫吸附测定结果显示，PE患者血清IL-8浓度（180.27±5.81 ng/L）显著高于健康对照组（41.57±5.67 ng/L）。重度PE患者血清IL-8水平更高。通过定量实时聚合酶链反应和免疫组织化学分析（轻度PE、重度PE和对照组各30例）证实了胎盘组织中IL-8相似的信使核糖核酸和蛋白质表达模式。此外，采用聚合酶链反应-限制性片段长度多态性/序列特异性引物检测IL-8基因的单核苷酸多态性。IL-8 -251A等位基因频率显著高于对照组（58.4%对48.9%，P<0.05）。PE患者中单体型-353A/-251A/+678T（AAT）的发生频率为27.2%，而对照组为21.9%（P<0.05）。我们的研究表明，IL-8表达与PE的严重程度呈正相关。孕妇中存在单体型AAT似乎是PE的一个危险因素。
