Xu Peng, Yu Qian, Huang Huizhen, Zhang Wen Jie, Li Wei
Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai 9th People's Hospital, Shanghai Jiao Tong University, 639 Zhi Zao Ju Road, Shanghai, 200011, China.
Aesthetic Plast Surg. 2018 Apr;42(2):343-351. doi: 10.1007/s00266-018-1091-4. Epub 2018 Jan 29.
To investigate the effects of nanofat injection into photoaged nude mouse skin on dermis thickness, neovascularization, and cell proliferation.
Adipose-derived stem cells (ADSCs) and nanofat were prepared from human liposuction aspirates. The photoaged skin model was created using ultraviolet B (UVB) radiation onto BALB/c nude mice. A total of 24 mice were used in this study; 6 mice without treatment (natural aging) served as controls, while 18 mice were irradiated under the UVB lamp and treated with PBS (200 μl per injection area), ADSCs (1 × 10/200 μl ADSCs per injection area), or nanofat (200 μl per injection area) on the dorsal skin. Four weeks after injection, skin specimens were collected. The skin texture of each group was evaluated by general observation. Histological analyses were performed to analyze skin structure, dermis thickness, collagen fiber arrangement, capillary density, and cell proliferation.
Four weeks after injection, no obvious differences were observed between the PBS group, ADSCs group, and nanofat group by skin gross observation. From the histological analyses, the ADSCs group and the nanofat group showed obviously thicker dermis than the PBS group (P < 0.05). More capillaries were observed in skin using anti-CD31 staining in the ADSCs and Nanofat groups than was observed in the PBS group (P < 0.05). No significant differences in the average dermis proliferation index were observed between groups by anti-Ki-67 staining. However, an increased epidermal proliferation index was observed in the ADSCs and Nanofat groups, compared to that in the PBS group (P < 0.05).
Nanofat increased dermis thickness and neovascularization in photoaged skin.
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探讨纳米脂肪注射到光老化裸鼠皮肤中对真皮厚度、新生血管形成及细胞增殖的影响。
从人抽脂吸出物中制备脂肪来源干细胞（ADSCs）和纳米脂肪。通过对BALB/c裸鼠进行紫外线B（UVB）照射建立光老化皮肤模型。本研究共使用24只小鼠；6只未接受治疗（自然衰老）的小鼠作为对照，18只小鼠在UVB灯下照射，并在背部皮肤注射PBS（每注射区域200μl）、ADSCs（每注射区域1×10/200μl ADSCs）或纳米脂肪（每注射区域200μl）。注射四周后，收集皮肤标本。通过大体观察评估每组的皮肤质地。进行组织学分析以分析皮肤结构、真皮厚度、胶原纤维排列、毛细血管密度和细胞增殖。
注射四周后，通过皮肤大体观察，PBS组、ADSCs组和纳米脂肪组之间未观察到明显差异。从组织学分析来看，ADSCs组和纳米脂肪组的真皮明显比PBS组厚（P<0.05）。与PBS组相比，ADSCs组和纳米脂肪组皮肤中抗CD31染色观察到的毛细血管更多（P<0.05）。抗Ki-67染色显示各组之间平均真皮增殖指数无显著差异。然而，与PBS组相比，ADSCs组和纳米脂肪组的表皮增殖指数增加（P<0.05）。
纳米脂肪增加了光老化皮肤的真皮厚度和新生血管形成。
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