Jeong Jae Hoon, Fan Yingfang, You Ga Young, Choi Tae Hyun, Kim Sukwha
Department of Plastic and Reconstructive Surgery, Institute of Human-Environment Interface Biology, Seoul National University College of Medicine, Seoul, South Korea.
Department of Plastic and Reconstructive Surgery, Institute of Human-Environment Interface Biology, Seoul National University College of Medicine, Seoul, South Korea; Department of Pediatric Plastic and Reconstructive Surgery, Seoul National University Children's Hospital, Seoul, South Korea.
J Plast Reconstr Aesthet Surg. 2015 Mar;68(3):372-81. doi: 10.1016/j.bjps.2014.10.045. Epub 2014 Nov 12.
We investigated the antiwrinkle effects of cultured human fibroblasts and adipose-derived stem cells (ADSCs) and the mechanisms underlying the reduction of wrinkles in photoaged skin. The fibroblasts and ADSCs were isolated from human tissue and cultured. A total of 28 6-week-old female BALB/c nude mice were classified into four groups, including the normal control group and three groups that were irradiated six times a week for 6-weeks using ultraviolet B radiation to induce photoaged wrinkles. ADSCs were injected into the wrinkles in the skin of the second group and fibroblasts were injected into the wrinkles in the skin of the third group. The fourth group was the irradiated negative control group (no therapy). After 4 weeks of injections, the wrinkles were compared by replica analysis, biopsies were performed, and the dermal thickness and collagen densities were measured. We determined the amounts of type 1 collagen and matrix metalloproteinases (MMPs) 1, 2, 3, 9, and 13 using real-time polymerase chain reaction and Western blot analysis, and we assessed tropoelastin and fibrillin-1 expression in the dermis by immunohistochemistry. Replica analysis showed significant wrinkle reduction in the fibroblast group and the ADSC group. ADSCs stimulated collagen expression and decreased MMP expression. Although fibroblasts stimulated more collagen expression than ADSCs, they also increased MMP expression. Overall, the ADSC group showed higher collagen density and had better outcomes in the tropoelastin and fibrillin-1 assessments. Both cultured fibroblasts and ADSCs could play an important role in wrinkle reduction despite differences in their mechanisms of action.
我们研究了培养的人成纤维细胞和脂肪来源干细胞（ADSCs）的抗皱效果以及光老化皮肤皱纹减少的潜在机制。从人体组织中分离并培养成纤维细胞和ADSCs。总共28只6周龄雌性BALB/c裸鼠被分为四组，包括正常对照组和三组每周接受6次紫外线B辐射共6周以诱导光老化皱纹的组。将ADSCs注射到第二组皮肤的皱纹中，将成纤维细胞注射到第三组皮肤的皱纹中。第四组为辐射阴性对照组（未治疗）。注射4周后，通过复制品分析比较皱纹情况，进行活检，并测量真皮厚度和胶原蛋白密度。我们使用实时聚合酶链反应和蛋白质印迹分析确定了I型胶原蛋白和基质金属蛋白酶（MMPs）1、2、3、9和13的含量，并通过免疫组织化学评估了真皮中原弹性蛋白和原纤维蛋白-1的表达。复制品分析显示成纤维细胞组和ADSC组的皱纹明显减少。ADSCs刺激胶原蛋白表达并降低MMP表达。虽然成纤维细胞比ADSCs刺激更多的胶原蛋白表达，但它们也增加了MMP表达。总体而言，ADSC组显示出更高的胶原蛋白密度，并且在原弹性蛋白和原纤维蛋白-1评估中具有更好的结果。尽管培养的成纤维细胞和ADSCs的作用机制不同，但它们在减少皱纹方面都可以发挥重要作用。
