Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology and Research, Singapore, Singapore.
Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, People's Republic of China.
J Ind Microbiol Biotechnol. 2018 Jul;45(7):491-516. doi: 10.1007/s10295-018-2004-x. Epub 2018 Jan 29.
In vivo biosensors can recognize and respond to specific cellular stimuli. In recent years, biosensors have been increasingly used in metabolic engineering and synthetic biology, because they can be implemented in synthetic circuits to control the expression of reporter genes in response to specific cellular stimuli, such as a certain metabolite or a change in pH. There are many types of natural sensing devices, which can be generally divided into two main categories: protein-based and nucleic acid-based. Both can be obtained either by directly mining from natural genetic components or by engineering the existing genetic components for novel specificity or improved characteristics. A wide range of new technologies have enabled rapid engineering and discovery of new biosensors, which are paving the way for a new era of biotechnological progress. Here, we review recent advances in the design, optimization, and applications of in vivo biosensors in the field of metabolic engineering and synthetic biology.
体内生物传感器可以识别和响应特定的细胞刺激。近年来，生物传感器在代谢工程和合成生物学中的应用越来越多，因为它们可以在合成回路中实现，以响应特定的细胞刺激来控制报告基因的表达，例如特定的代谢物或 pH 值的变化。有许多类型的天然感应装置，它们通常可以分为两类：基于蛋白质的和基于核酸的。这两者都可以通过直接从天然遗传成分中挖掘，或者通过工程改造现有遗传成分来获得，以获得新的特异性或改进的特性。广泛的新技术使快速工程和发现新的生物传感器成为可能，为生物技术进步的新时代铺平了道路。在这里，我们综述了代谢工程和合成生物学领域中体内生物传感器的设计、优化和应用的最新进展。
