National Agricultural Research Center for Tohoku Region, NARO, Morioka, Iwate, 020-0198, Japan.
Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980 - 0845, Japan.
Sci Rep. 2018 Jan 30;8(1):1870. doi: 10.1038/s41598-018-19527-3.
Tocopherol (Toc) and tocotrienol (T3) are abundant in rice bran. Geranylgeranyl reductase (GGR) is an essential enzyme for Toc production that catalyzes the reduction of geranylgeranyl pyrophosphate and geranylgeranyl-chlorophyll. However, we found that a rice mutant line with inactivated Os02g0744900 (OsGGR1/LYL1/OsChl P) gene produces Toc, suggesting that rice plants may carry another enzyme with GGR activity. Using an RNA-mediated interference technique, we demonstrated that the Os01g0265000 ("OsGGR2") gene product has GGR activity. This result supports the existence of two GGR genes (OsGGR1 and OsGGR2) in rice, in contrast to Arabidopsis thaliana (thale cress) and cyanobacterium Synechocystis that each have only one GGR gene. We also produced rice callus with inactivated OsGGR1 and OsGGR2 that produced T3 but not Toc. Such rice callus could be used as a resource for production of pure T3 for nutraceutical applications.
生育酚（Toc）和三烯生育酚（T3）在米糠中含量丰富。香叶基香叶基焦磷酸还原酶（GGR）是 Toc 生产的必需酶，它催化香叶基香叶基焦磷酸和香叶基叶绿素的还原。然而，我们发现一个 Os02g0744900（OsGGR1/LYL1/OsChlP）基因失活的水稻突变体系也能产生 Toc，这表明水稻可能携带另一种具有 GGR 活性的酶。利用 RNA 介导的干扰技术，我们证明了 Os01g0265000（“OsGGR2”）基因产物具有 GGR 活性。这一结果支持了水稻中存在两个 GGR 基因（OsGGR1 和 OsGGR2），而拟南芥和蓝藻 Synechocystis 每个只有一个 GGR 基因。我们还生产了 OsGGR1 和 OsGGR2 失活的水稻愈伤组织，它们能产生 T3 但不能产生 Toc。这种水稻愈伤组织可以作为生产用于营养保健品的纯 T3 的资源。
Zotero 插件
