He Fang, Shi Yu-Jie, Chen Qi, Li Jun-Lin, Niu Meng-Xue, Feng Cong-Hua, Lu Meng-Meng, Tian Fei-Fei, Zhang Fan, Lin Tian-Tian, Chen Liang-Hua, Liu Qin-Lin, Wan Xue-Qin
Sichuan Province Key Laboratory of Ecological Forestry Engineering on the Upper Reaches of the Yangtze River, College of Forestry, Sichuan Agricultural University, Chengdu, China.
College of Landscape Architecture, Sichuan Agricultural University, Chengdu, China.
Front Plant Sci. 2022 May 10;13:870970. doi: 10.3389/fpls.2022.870970. eCollection 2022.
Chlorophyll (Chl) plays a crucial role in plant photosynthesis. The geranylgeraniol reductase gene () participates in the terminal hydrogenation of chlorophyll biosynthesis. Although there are many studies related to the genome-wide analysis of , little research has been conducted on family genes, especially those concerning growth and photosynthesis. In this study, three genes were identified in . The evolutionary tree indicated that the family genes were divided into six groups. Moreover, one pair of genes was derived from segmental duplications in . Many elements related to growth were detected by -acting element analysis of the promoters of diverse . Furthermore, exhibit different tissue expression patterns. In addition, is preferentially expressed in the leaves and plays an important role in regulating chlorophyll biosynthesis. Silencing of in poplar resulted in a decrease in chlorophyll synthesis in plants, thus blocking electron transport during photosynthesis. Furthermore, inhibition of expression in poplar can inhibit plant growth through the downregulation of photosynthesis. Ultimately, formed a co-expression network with photosynthesis and chlorophyll biosynthesis-related genes, which synergistically affected the growth and photosynthesis of poplars. Thus, this study provides genetic resources for the improved breeding of fast-growing tree traits.
叶绿素（Chl）在植物光合作用中起着至关重要的作用。香叶基香叶醇还原酶基因（）参与叶绿素生物合成的末端氢化过程。尽管有许多关于的全基因组分析的研究，但对家族基因的研究较少，尤其是那些与生长和光合作用相关的基因。在本研究中，在中鉴定出了三个基因。进化树表明家族基因被分为六组。此外，一对基因源自中的片段重复。通过对不同基因启动子的顺式作用元件分析，检测到许多与生长相关的元件。此外，表现出不同的组织表达模式。另外，在叶片中优先表达，并在调节叶绿素生物合成中起重要作用。杨树中的沉默导致植物叶绿素合成减少，从而阻断光合作用过程中的电子传递。此外，杨树中表达的抑制可通过光合作用的下调来抑制植物生长。最终，与光合作用和叶绿素生物合成相关基因形成了共表达网络，协同影响杨树的生长和光合作用。因此，本研究为速生树木性状的改良育种提供了遗传资源。
