Safaie-Farahani Ali, Taghavi S Mohsen
Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, Iran.
Mol Biol Res Commun. 2017 Dec;6(4):177-183. doi: 10.22099/mbrc.2017.25600.1273.
The transcript levels of six defense genes including pathogenesis-related gene 1 (), pathogenesis-related gene 2 (), pathogenesis-related gene 5 (), lipoxygenase (), phenylalanine ammonia-lyase () and catalase () were investigated in tomato plants inoculated with pv. as a non-host pathogen and as a host pathogen. Activation of all the genes was confirmed in both host and non-host treatments. Additionally, the results showed stronger expression of majority of the genes (, , , and ) in non-host treatment compared to host treatment at least at early hours after inoculation. These data suggest that faster and more expression of , , , and might have a role in non-host resistance of tomato against pv. .
在接种丁香假单胞菌番茄致病变种(Pseudomonas syringae pv. tomato)作为非寄主病原菌以及番茄灰霉病菌(Botrytis cinerea)作为寄主病原菌的番茄植株中,研究了六个防御基因的转录水平,这些基因包括病程相关蛋白基因1(pathogenesis-related gene 1)、病程相关蛋白基因2(pathogenesis-related gene 2)、病程相关蛋白基因5(pathogenesis-related gene 5)、脂氧合酶(lipoxygenase)、苯丙氨酸解氨酶(phenylalanine ammonia-lyase)和过氧化氢酶(catalase)。在寄主和非寄主处理中均证实了所有基因的激活。此外,结果表明,至少在接种后的早期,与寄主处理相比,大多数基因(病程相关蛋白基因1、病程相关蛋白基因2、病程相关蛋白基因5、脂氧合酶和苯丙氨酸解氨酶)在非寄主处理中的表达更强。这些数据表明,病程相关蛋白基因1、病程相关蛋白基因2、病程相关蛋白基因5、脂氧合酶和苯丙氨酸解氨酶更快且更多的表达可能在番茄对丁香假单胞菌番茄致病变种的非寄主抗性中发挥作用。