INRA, UMR 1347 Agroecologie, Dijon, France.
Mol Plant Microbe Interact. 2013 Aug;26(8):918-26. doi: 10.1094/MPMI-12-12-0290-R.
The protective Fusarium oxysporum strain Fo47 is effective in controlling Fusarium wilt in tomato. Previous studies have demonstrated the role of direct antagonism and involvement of induced resistance. The aim of the present study was to investigate whether priming of plant defense responses is a mechanism by which Fo47 controls Fusarium wilt. An in vitro design enabled inoculation of the tap root with Fo47 and the pathogenic strain (Fol8) at different locations and different times. The expression levels of six genes known to be involved in tomato defense responses were quantified using reverse-transcription quantitative polymerase chain reaction (qPCR). Three genes-CHI3, GLUA, and PR-1a-were overexpressed in the root preinoculated with Fo47, and then challenged with Fol8. The genes GLUA and PR-1a were upregulated in cotyledons after inoculation of Fo47. Fungal growth in the root was assessed by qPCR, using specific markers for Fo47 and Fol8. Results showed a reduction of the pathogen growth in the root of the tomato plant preinoculated with Fo47. This study demonstrated that priming of tomato defense responses is one of the mechanisms of action of Fo47, which induces a reduced colonization of the root by the pathogen.
保护性的尖孢镰刀菌菌株 Fo47 可有效防治番茄枯萎病。先前的研究表明，其作用机制包括直接拮抗和诱导抗性的参与。本研究旨在探讨 Fo47 防控枯萎病是否通过植物防御反应的激发来实现。通过体外设计，可在不同位置和不同时间将 Fo47 和病原菌（Fol8）接种到番茄的主根上。采用反转录定量聚合酶链反应（qPCR）技术，对 6 个已知参与番茄防御反应的基因的表达水平进行了定量分析。Fo47 预接种后，CHI3、GLUA 和 PR-1a 这 3 个基因在根中过表达，然后再用 Fol8 进行挑战。Fo47 接种后，GLUA 和 PR-1a 这 2 个基因在子叶中上调表达。通过 Fo47 和 Fol8 的特异性标记物，采用 qPCR 对根中的真菌生长进行了评估。结果表明，Fo47 预接种可减少番茄植株根中病原菌的生长。本研究表明，番茄防御反应的激发是 Fo47 作用机制之一，其可诱导病原菌对根的定殖减少。
