Neshastehriz Ali, Khateri Maziar, Ghaznavi Habib, Shakeri-Zadeh Ali
Radiation Biology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran.
Radiation Sciences Department, Iran University of Medical Sciences (IUMS), Tehran, Iran.
Anticancer Agents Med Chem. 2018;18(6):882-890. doi: 10.2174/1871520618666180131112914.
It has been well-known both gold nanoparticles (AuNPs) and cisplatin are potential radiosensitizers for radiotherapy of cancer. In this in vitro study, we investigated the chemoradiotherapeutic effects of alginate nanogel co-loaded with AuNPs and cisplatin (ACA) on U87-MG human glioblastoma cells.
Based on the accomplished pilot studies, U87-MG cells were incubated with ACA and cisplatin at 10% inhibitory concentration (IC10) for 4h. Then, the cells were irradiated to different doses of 6MV X-rays (2 and 10 Gy). MTT assay was performed to evaluate the cell survival rate. Apoptosis was determined by flow cytometry using an annexinV-fluorescein isothiocyanate/propidium iodide apoptosis detection kit.
The results showed that ACA at the concentration of 4 µg/ml (per cisplatin) and free cisplatin at concentration of 15 μg/ml have the same effects on U87-MG cells (survival rate: 90%). The combination of ACA with radiation resulted in a significant decrease in cell viability (survival rate: 30%). The flow cytometry assay also showed that such a combination therapy induces more apoptosis than necrosis.
It may be concluded that co-delivery of AuNPs and cisplatin with a single nanoplatform like ACA nanocomplex enhances the therapeutic ratio of human glioblastoma radiation therapy.
众所周知，金纳米颗粒（AuNPs）和顺铂都是癌症放射治疗的潜在放射增敏剂。在这项体外研究中，我们研究了负载AuNPs和顺铂的海藻酸钠纳米凝胶（ACA）对U87-MG人胶质母细胞瘤细胞的放化疗效果。
基于已完成的初步研究，将U87-MG细胞与ACA和顺铂以10%抑制浓度（IC10）孵育4小时。然后，将细胞照射不同剂量的6MV X射线（2和10 Gy）。进行MTT试验以评估细胞存活率。使用膜联蛋白V-异硫氰酸荧光素/碘化丙啶凋亡检测试剂盒通过流式细胞术测定细胞凋亡。
结果表明，浓度为4μg/ml（每顺铂）的ACA和浓度为15μg/ml的游离顺铂对U87-MG细胞具有相同的作用（存活率：90%）。ACA与放疗联合导致细胞活力显著降低（存活率：30%）。流式细胞术检测还表明，这种联合治疗诱导的凋亡多于坏死。
可以得出结论，像ACA纳米复合物这样的单一纳米平台共同递送AuNPs和顺铂可提高人胶质母细胞瘤放射治疗的治疗比率。
