1 Department of Medical Physics, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran.
2 Radiation Sciences Department, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
J Biomater Appl. 2018 Aug;33(2):161-169. doi: 10.1177/0885328218782355. Epub 2018 Jun 22.
The biomedical applications of gold nanoparticles (AuNPs) have experienced rapid growth in recent years, due to their expected benefits in medical imaging and therapy. In this work, we report the development of a theranostic nanocomplex constructed from alginate hydrogel co-loaded with cisplatin and AuNPs (abbreviated as ACA) for simultaneous drug delivery and computed tomography imaging. CT26 cells derived from mouse colon adenocarcinoma were exposed to various concentrations of ACA nanocomplex (for 24 h) and the cytotoxicity was measured using MTT assay. Moreover, the cells treated with ACA nanocomplex were imaged in a computed tomography scanner and the contrast enhancement due to the presence of nanocomplex was assessed. The cytotoxicity results showed that ACA nanocomplex had a more potent chemotherapy efficacy than free cisplatin, so that ACA nanocomplex at the concentration of 5 µg/ml (per cisplatin) and 20 µg/ml of free cisplatin resulted in the same cytotoxicity (survival rate: 66%). The computed tomography imaging study revealed that ACA nanocomplex increased the brightness of computed tomography images, the computed tomography number value, and contrast-to-noise ratio (CNR). ACA nanocomplex can be presented as a computed tomography-traceable nanocarrier that allows to monitor the delivery of therapeutics by assessing their localized accumulation and in vivo biodistribution.
近年来，由于金纳米粒子（AuNPs）在医学成像和治疗方面的预期益处，其在生物医学中的应用迅速发展。在这项工作中，我们报告了一种由载顺铂和 AuNPs 的海藻酸钠水凝胶共载体制成的治疗诊断纳米复合物（简称 ACA）的开发，用于药物递释和计算机断层扫描成像。将源自小鼠结肠腺癌的 CT26 细胞暴露于不同浓度的 ACA 纳米复合物（24 h），并通过 MTT 测定法测量细胞毒性。此外，在计算机断层扫描扫描仪中对用 ACA 纳米复合物处理的细胞进行成像，并评估由于纳米复合物的存在而产生的对比增强。细胞毒性结果表明，ACA 纳米复合物比游离顺铂具有更强的化疗功效，因此 ACA 纳米复合物在 5 µg/ml（每顺铂）和 20 µg/ml 游离顺铂的浓度下产生相同的细胞毒性（存活率：66%）。计算机断层扫描成像研究表明，ACA 纳米复合物增加了计算机断层扫描图像的亮度、计算机断层扫描数值值和对比噪声比（CNR）。ACA 纳米复合物可以作为一种计算机断层扫描可追踪的纳米载体，通过评估其局部积累和体内分布来监测治疗药物的递送。
