使用超分辨率显微镜探针实时追踪细胞内细胞器中的次氯酸浓度。
Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe.
作者信息
Ali Firoj, Aute Sunil, Sreedharan Sreejesh, Anila H A, Saeed Hiwa K, Smythe Carl G, Thomas Jim A, Das Amitava
机构信息
Organic Chemistry Division, CSIR-National Chemical Laboratory, Pune - 411008, India.
出版信息
Chem Commun (Camb). 2018 Feb 15;54(15):1849-1852. doi: 10.1039/c7cc09433g.
BODIPY derivative, SF-1, exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles. SF-1 is compatible with 3D-SIM imaging of individual cells.
硼二吡咯亚甲基衍生物SF-1仅对次氯酸显示荧光开启响应，并能对RAW 264.7巨噬细胞内源性产生的次氯酸进行成像。宽场和超分辨率结构照明显微镜图像证实其定位于高尔基体复合体和溶酶体，因此能特异性检测这些细胞器中产生的次氯酸。SF-1与单个细胞的三维结构照明显微成像兼容。
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