Department of Biomedical Engineering, University at Buffalo, State University of New York , Buffalo, New York 14260, United States.
Nano Lett. 2018 Feb 14;18(2):1331-1336. doi: 10.1021/acs.nanolett.7b05025. Epub 2018 Feb 2.
Many approaches exist for stimuli-triggered cargo release from nanocarriers, but few can provide for on-demand release of multiple payloads, selectively. Here, we report the synthesis of purpurin-phospholipid (Pur-P), a lipid chromophore that has near-infrared absorbance red-shifted by 30 nm compared to a structurally similar pyropheophorbide-phospholipid (Pyr-P). Liposomes containing small amounts of either Pur-P or Pyr-P exhibited similar physical properties and fluorescence self-quenching. Loaded with distinct cargos, Pur-P and Pyr-P liposomes were mixed into a single colloidal suspension and selectively released cargo depending on irradiation wavelength. Spatiotemporal control of distinct cargo release was achieved by controlling multicolor laser placement. Using basic orange and doxorubicin anthraquinones, multidimensional cytotoxicity gradients were established to gauge efficacy against cancer cells using light-released drug. Wavelength selectivity of cargo release was maintained following intramuscular administration to mice.
许多方法可用于实现纳米载体中货物的刺激触发释放，但很少有方法能够按需选择性地释放多种有效载荷。在这里，我们报告了卟啉磷脂（Pur-P）的合成，与结构相似的焦脱镁叶绿酸磷脂（Pyr-P）相比，Pur-P 的近红外吸收红移了 30nm。含有少量 Pur-P 或 Pyr-P 的脂质体表现出相似的物理性质和荧光自猝灭。负载不同货物的 Pur-P 和 Pyr-P 脂质体混合到单个胶体悬浮液中，并根据照射波长选择性释放货物。通过控制多色激光放置，实现了对不同货物释放的时空控制。使用碱性橙和阿霉素蒽醌，建立了多维细胞毒性梯度，以用光释放的药物来评估其对癌细胞的疗效。在向小鼠肌肉内给药后，仍能保持货物释放的波长选择性。
