Nevozhay Dmitry, Weiger Michael, Friedl Peter, Sokolov Konstantin V
Department of Imaging Physics, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA.
School of Biomedicine, Far Eastern Federal University, 8 Sukhanova Street, Vladivostok, 690950, Russia.
Biomed Opt Express. 2019 Jun 13;10(7):3301-3316. doi: 10.1364/BOE.10.003301. eCollection 2019 Jul 1.
Here, we present a new class of third harmonic generation (THG) imaging probes that can be activated with precise spatiotemporal control using non-linear excitation. These probes consist of lipid-coated perfluorocarbon nanodroplets with embedded visible chromophores. The droplets undergo phase transition from liquid to gas upon heating mediated by two-photon absorption of NIR light by the embedded dyes. Resulting microbubbles provide a sharp, local refractive index mismatch, which makes an excellent source of THG signal. Potential applications of these probes include activatable THG agents for biological imaging and "on-demand" delivery of various compounds under THG monitoring.
在此，我们展示了一类新型的三次谐波产生（THG）成像探针，其可通过非线性激发实现精确的时空控制激活。这些探针由包裹脂质的全氟化碳纳米液滴组成，其中嵌入了可见发色团。在近红外光被嵌入染料双光子吸收介导的加热作用下，液滴经历从液体到气体的相变。产生的微泡提供了明显的、局部的折射率失配，这使其成为THG信号的极佳来源。这些探针的潜在应用包括用于生物成像的可激活THG试剂，以及在THG监测下“按需”递送各种化合物。
