Pagano Imma, Sánchez-Camargo Andrea Del Pilar, Mendiola Jose Antonio, Campone Luca, Cifuentes Alejandro, Rastrelli Luca, Ibañez Elena
Department of Pharmacy, University of Salerno, Fisciano, SA, Italy.
Ph.D. Program in Drug Discovery and Development, University of Salerno, Fisciano, SA, Italy.
Electrophoresis. 2018 Jan 31. doi: 10.1002/elps.201700442.
During the essential oil steam distillation from aromatic herbs, huge amounts of distillation wastewaters (DWWs) are generated. These by-products represent an exceptionally rich source of phenolic compounds such as rosmarinic acid (RA) and caffeic acid (CA). Herein, the alternative use of dried basil DWWs (dDWWs) to perform a selective extraction of RA and CA by pressurized liquid extraction (PLE) employing bio-based solvent was studied. To select the most suitable solvent for PLE, the theoretical modelling of Hansen solubility parameters (HSP) was carried out. This approach allows reducing the list of candidate to two solvents: ethanol and ethyl lactate. Due to the composition of the sample, mixtures of water with those solvents were also tested. An enriched PLE extract in RA (23.90 ± 2.06 mg/g extract) with an extraction efficiency of 75.89 ± 16.03% employing a water-ethanol mixture 25:75 (% v/v) at 50°C was obtained. In the case of CA, a PLE extract with 2.42 ± 0.04 mg/g extract, having an extraction efficiency of 13.86 ± 4.96% using ethanol absolute at 50°C was achieved. DWWs are proposed as new promising sources of natural additives and/or functional ingredients for cosmetic, nutraceutical, and food applications.
在从芳香草本植物中进行精油水蒸气蒸馏的过程中，会产生大量的蒸馏废水（DWWs）。这些副产品是酚类化合物的极为丰富的来源，如迷迭香酸（RA）和咖啡酸（CA）。在此，研究了利用干燥罗勒蒸馏废水（dDWWs），采用生物基溶剂通过加压液体萃取（PLE）选择性萃取RA和CA。为了选择最适合PLE的溶剂，进行了汉森溶解度参数（HSP）的理论建模。这种方法使候选溶剂名单减少到两种：乙醇和乳酸乙酯。由于样品的组成，还测试了水与这些溶剂的混合物。在50℃下使用25:75（% v/v）的水 - 乙醇混合物，获得了富含RA的PLE提取物（23.90±2.06 mg/g提取物），萃取效率为75.89±16.03%。对于CA，在50℃下使用无水乙醇，获得了含量为2.42±0.04 mg/g提取物的PLE提取物，萃取效率为13.86±4.96%。蒸馏废水被认为是化妆品、营养保健品和食品应用中天然添加剂和/或功能成分的新的有前景的来源。
