Department of Biology & BK21- Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University, Gwangju 61452, Korea.
Mol Cells. 2018 Feb 28;41(2):103-109. doi: 10.14348/molcells.2018.2170. Epub 2018 Jan 29.
Calcium ions are involved in the regulation of diverse cellular processes. Fourteen genes encoding calcium binding proteins have been identified in . CBP7, one of the 14 CBPs, is composed of 169 amino acids and contains four EF-hand motifs. Here, we investigated the roles of CBP7 in the development and cell migration of cells and found that high levels of CBP7 exerted a negative effect on cells aggregation during development, possibly by inhibiting chemoattractant-directed cell migration. While cells lacking CBP7 exhibited normal development and chemotaxis similar that of wild-type cells, CBP7 overexpressing cells completely lost their chemotactic abilities to move toward increasing cAMP concentrations. This resulted in inhibition of cellular aggregation, a process required for forming multicellular organisms during development. Low levels of cytosolic free calcium were observed in CBP7 overexpressing cells, which was likely the underlying cause of their lack of chemotaxis. Our results demonstrate that CBP7 plays an important role in cell spreading and cell-substrate adhesion. null cells showed decreased cell size and cell-substrate adhesion. The present study contributes to further understanding the role of calcium signaling in regulation of cell migration and development.
钙离子参与调节多种细胞过程。在 中已经鉴定出 14 种编码钙结合蛋白的基因。CBP7 是这 14 种 CBPs 之一，由 169 个氨基酸组成，包含四个 EF 手模体。在这里，我们研究了 CBP7 在 细胞发育和细胞迁移中的作用，发现高水平的 CBP7 对发育过程中细胞聚集产生负面影响，可能通过抑制趋化因子定向的细胞迁移。而缺乏 CBP7 的细胞表现出正常的发育和趋化性，与野生型细胞相似，但过表达 CBP7 的细胞完全丧失了向 cAMP 浓度增加的趋化能力。这导致细胞聚集受到抑制，这是发育过程中形成多细胞生物体所必需的过程。在过表达 CBP7 的细胞中观察到细胞浆游离钙水平较低，这可能是它们缺乏趋化性的潜在原因。我们的结果表明，CBP7 在细胞铺展和细胞-基质黏附中发挥重要作用。 缺失细胞表现出细胞变小和细胞-基质黏附减少。本研究有助于进一步了解钙信号在调节细胞迁移和发育中的作用。
