Department of Biology, College of Natural Sciences, Chosun University, Gwangju 501-759, Korea.
Mol Cells. 2010 Dec;30(6):563-7. doi: 10.1007/s10059-010-0153-5. Epub 2010 Nov 18.
Rap1 is rapidly activated upon chemoattractant stimulation and plays an important role in cell adhesion and cytoskeletal reorganization during chemotaxis. Here, we demonstrate that G-protein coupled receptors and G-proteins are essential for chemoattractant-mediated Rap1 activation in Dictyostelium. The rapid Rap1 activation upon cAMP chemoattractant stimulation was absent in cells lacking chemoattractant cAMP receptors cAR1/cAR3 or a subunit of the heterotrimeric G-protein complex Gα2. Loss of guanylyl cyclases GCA/SGC or a cGMP-binding protein GbpC exhibited no effect on Rap1 activation kinetics. These results suggest that Rap1, a key regulator for the regulation of cytoskeletal reorganization during cell movement, is activated through the G-protein coupled receptors cAR1/cAR3 and Gα2 proteins in a way independent of the cGMP signaling pathway.
Rap1 在趋化刺激下迅速激活，在趋化过程中细胞黏附和细胞骨架重排中发挥重要作用。本文作者证明，G 蛋白偶联受体和 G 蛋白对于 Dd 细胞中趋化因子介导的 Rap1 激活是必需的。在缺乏趋化因子 cAMP 受体 cAR1/cAR3 或异三聚体 G 蛋白复合物 Gα2 的亚基的细胞中，cAMP 趋化刺激引起的 Rap1 快速激活缺失。鸟苷酸环化酶 GCA/SGC 或 cGMP 结合蛋白 GbpC 的缺失对 Rap1 激活动力学没有影响。这些结果表明，Rap1 是细胞运动过程中细胞骨架重排的关键调节剂，通过 G 蛋白偶联受体 cAR1/cAR3 和 Gα2 蛋白激活，而不依赖于 cGMP 信号通路。
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