Abdallah Hossam M, Asfour Hani Z, El-Halawany Ali M, Elfaky Mahmoud A
Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia / Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
Pak J Pharm Sci. 2018 Jan;31(1(Suppl.)):325-332.
This study was performed to assess the potential β-lactamase inhibitory properties of nineteen crude Saudi plant extracts belonging to eight families against extended spectrum β-lactamase (ESβL) strains of Klebsiella pneumoniae and other medically important pathogens. A total of 276 microbial isolates of pathogenic bacteria were used in this study; only 15 of them showed decreased sensitivity to one or several of ceftazidime, aztreonam, cefotaxime or ceftriaxone, which are deemed to be possible producers of ESβL. Antibacterial activities of plant extracts were carried out against ESβL positive isolates by the disc diffusion method. The potential ESβL suppressing activities of plant extracts and prepared fractions, (chloroform and methanol), with or without antibiotic were studied by disc diffusion method. Results revealed that selected plant extracts showed no antibacterial activity against tested strains; meanwhile, only Echinops viscosus, Pulicaria arabica, Tephrosia nubica, Chrozophora oblongifolia, and Clutia myricoides showed pronounced ESβL inhibitory activities. The extracts were quantified for phenolic compounds and their antioxidant properties. Bio-guided fractionation of the active extracts revealed that the chloroform fraction of C. myricoides possess a promising ESβL inhibitory activity. The separation and the structural elucidation of the active compounds from C. myricoides will offer beneficial leads for developing β-lactamase inhibitors.
本研究旨在评估来自沙特八个科的19种植物粗提物对肺炎克雷伯菌的超广谱β-内酰胺酶（ESβL）菌株及其他医学上重要病原体的潜在β-内酰胺酶抑制特性。本研究共使用了276株病原菌微生物分离株；其中只有15株对头孢他啶、氨曲南、头孢噻肟或头孢曲松中的一种或几种表现出敏感性降低，这些菌株被认为可能是ESβL的产生菌。采用纸片扩散法测定植物提取物对ESβL阳性分离株的抗菌活性。通过纸片扩散法研究了植物提取物及其制备的馏分（氯仿和甲醇）在有或无抗生素存在下的潜在ESβL抑制活性。结果显示，所选植物提取物对受试菌株无抗菌活性；同时，只有粘性蓝刺头、阿拉伯蚤草、努比亚灰毛豆、长圆叶地锦和杨梅叶克卢西亚显示出明显的ESβL抑制活性。对提取物中的酚类化合物及其抗氧化性能进行了定量分析。对活性提取物进行生物导向分离，结果表明杨梅叶克卢西亚的氯仿馏分具有良好的ESβL抑制活性。从杨梅叶克卢西亚中分离和鉴定活性化合物将为开发β-内酰胺酶抑制剂提供有益线索。
