Quintana Ramiro Martins, Jardine Alexander Pompermayer, Montagner Francisco, Fatturi Parolo Clarissa Cavalcanti, Morgental Renata Dornelles, Poli Kopper Patrícia Maria
Graduate program in dentistry, Faculty of Dentistry, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil.
Department of Social and Preventive Dentistry, Faculty of Dentstry, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil.
J Conserv Dent. 2017 Sep-Oct;20(5):341-345. doi: 10.4103/JCD.JCD_129_16.
Human dentin powder (HD), bovine serum albumin (BSA) and endotoxin (LPS) may affect the antimicrobial activity of irrigating solutions.
To evaluate the inhibitory effect of HD powder, BSA, and LPS on the antibacterial activity of 0.5%, 1%, 2.5%, and 5% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) gel, BioPure mixture of tetracycline, citric acid, and detergent (MTAD), and QMix.
The direct contact test against (ATCC 29212) for 2-min, 30-min and 6-h was used. Sterile pyrogen-free water was the negative control. After experimental periods, a neutralizing agent was used. Colony-forming units were determined by 10-fold serial dilutions and culture on agar plates. Data were analyzed by Kruskal-Wallis and Dunn's test (α = 5%).
In the absence of inhibitors, all irrigants eliminated . In contact with HD, all solutions eliminated within 2-min, with the exception of MTAD. In the presence of BSA, only 5% NaOCl killed within 2-min. LPS did not affect the antibacterial effect of any irrigant. At 30-min and at 6-h, all substances eliminated .
In the presence of albumin, irrigants needed >2-min to eliminate , except for 5% NaOCl. The same was observed in the presence of dentin when was exposed to MTAD.
人牙本质粉（HD）、牛血清白蛋白（BSA）和内毒素（LPS）可能会影响冲洗液的抗菌活性。
评估HD粉、BSA和LPS对0.5%、1%、2.5%和5%次氯酸钠（NaOCl）、2%氯己定（CHX）凝胶、四环素、柠檬酸和去污剂的BioPure混合物（MTAD）以及QMix抗菌活性的抑制作用。
采用对粪肠球菌（ATCC 29212）进行2分钟、30分钟和6小时的直接接触试验。无菌无热原水为阴性对照。在实验期后，使用中和剂。通过10倍系列稀释并在琼脂平板上培养来确定菌落形成单位。数据采用Kruskal-Wallis和Dunn检验进行分析（α = 5%）。
在没有抑制剂的情况下，所有冲洗液都能消除粪肠球菌。与HD接触时，除MTAD外，所有溶液在2分钟内都能消除粪肠球菌。在存在BSA的情况下，只有5%的NaOCl在2分钟内杀死粪肠球菌。LPS不影响任何冲洗液的抗菌效果。在30分钟和6小时时，所有物质都能消除粪肠球菌。
在存在白蛋白的情况下，除5%的NaOCl外，冲洗液需要超过2分钟才能消除粪肠球菌。当粪肠球菌暴露于MTAD时，在存在牙本质的情况下也观察到了同样的情况。
