外泌体介导的MALAT1递送诱导乳腺癌细胞增殖。
Exosome-mediated delivery of MALAT1 induces cell proliferation in breast cancer.
作者信息
Zhang Ping, Zhou Hongxing, Lu Kefeng, Lu Yunou, Wang Yan, Feng Tongbao
机构信息
Department of Clinical Laboratory, The Affiliated Changzhou No 2 People's Hospital, Nanjing Medical University, Changzhou, People's Republic of China.
出版信息
Onco Targets Ther. 2018 Jan 9;11:291-299. doi: 10.2147/OTT.S155134. eCollection 2018.
BACKGROUND
Breast cancer is the most common cancer in women worldwide. Cancer-secreted exosomes have recently been recognized as important mediators of intercellular communication. The aim of this study was to determine the role of exosomal long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in breast cancer progression.
MATERIALS AND METHODS
Breast cancer specimens were obtained with informed consent from patients. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect MALAT1 expression, and cellular proliferation was measured using cell counting kit-8 (CCK-8) assay.
RESULTS
MALAT1 was highly expressed in breast cancer tissues and associated with disease progression. Breast cancer exosomes promoted cell proliferation and exosome-mediated MALAT1 to induce cell proliferation.
CONCLUSION
These findings indicated that exosomal MALAT1 could regulate cancer progression and represent a novel strategy for overcoming breast cancer.
背景
乳腺癌是全球女性中最常见的癌症。癌症分泌的外泌体最近被认为是细胞间通讯的重要介质。本研究的目的是确定外泌体长链非编码RNA转移相关肺腺癌转录本1（MALAT1）在乳腺癌进展中的作用。
材料与方法
在获得患者知情同意后获取乳腺癌标本。采用定量实时聚合酶链反应（qRT-PCR）检测MALAT1表达，并使用细胞计数试剂盒-8（CCK-8）检测法测量细胞增殖。
结果
MALAT1在乳腺癌组织中高表达，并与疾病进展相关。乳腺癌外泌体促进细胞增殖，且外泌体介导的MALAT1诱导细胞增殖。
结论
这些发现表明外泌体MALAT1可调节癌症进展，并代表了一种克服乳腺癌的新策略。
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