Zhang Ping, Zhou Hongxing, Lu Kefeng, Lu Yunou, Wang Yan, Feng Tongbao
Department of Clinical Laboratory, The Affiliated Changzhou No 2 People's Hospital, Nanjing Medical University, Changzhou, People's Republic of China.
Onco Targets Ther. 2018 Jan 9;11:291-299. doi: 10.2147/OTT.S155134. eCollection 2018.
Breast cancer is the most common cancer in women worldwide. Cancer-secreted exosomes have recently been recognized as important mediators of intercellular communication. The aim of this study was to determine the role of exosomal long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in breast cancer progression.
Breast cancer specimens were obtained with informed consent from patients. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect MALAT1 expression, and cellular proliferation was measured using cell counting kit-8 (CCK-8) assay.
MALAT1 was highly expressed in breast cancer tissues and associated with disease progression. Breast cancer exosomes promoted cell proliferation and exosome-mediated MALAT1 to induce cell proliferation.
These findings indicated that exosomal MALAT1 could regulate cancer progression and represent a novel strategy for overcoming breast cancer.
乳腺癌是全球女性中最常见的癌症。癌症分泌的外泌体最近被认为是细胞间通讯的重要介质。本研究的目的是确定外泌体长链非编码RNA转移相关肺腺癌转录本1（MALAT1）在乳腺癌进展中的作用。
在获得患者知情同意后获取乳腺癌标本。采用定量实时聚合酶链反应（qRT-PCR）检测MALAT1表达，并使用细胞计数试剂盒-8（CCK-8）检测法测量细胞增殖。
MALAT1在乳腺癌组织中高表达，并与疾病进展相关。乳腺癌外泌体促进细胞增殖，且外泌体介导的MALAT1诱导细胞增殖。
这些发现表明外泌体MALAT1可调节癌症进展，并代表了一种克服乳腺癌的新策略。
