Eligio-García Leticia, Pontifez-Pablo Elida, Pérez-Gutiérrez Salúd, Jiménez-Cardoso Enedina
Parasitology Research Laboratory, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Col. Doctores, Delegación Cuauhtémoc, 06720 Ciudad de México, Mexico.
Biological Systems Department, UAM Xochimilco, Calzada del Hueso 1100, Col. Villa Quietud, Delegación Coyoacán, 04960 Ciudad de México, Mexico.
Evid Based Complement Alternat Med. 2017;2017:6832789. doi: 10.1155/2017/6832789. Epub 2017 Dec 13.
A variety of drugs are used in giardiasis treatment with different levels of efficiency, presence of side effects, and even formation of resistant strains, so that it is important to search new only-one-dose treatments with high efficiency and less side effects. Kramecyne, an anti-inflammatory compound isolated from methanolic extract of , does not present toxicity, even at doses of 5,000 mg/kg. The objective was to determine the antigiardial effect of kramecyne over and and analyze the expression of genes ERK1, ERK2, and AK on kramecyne treated trophozoites by Real Time Polymerase Chain Reaction (RTPCR). The median lethal dose (LD) was 40 g/mL and no morphological changes were observed by staining with blue trypan and light microscopy; experimental gerbil infection was eliminated with 320 g/Kg of weight. After treatment there were no differences between intestines from treated and untreated gerbils. Kramecyne did not have significant effect over ERK1 and AK, but there are differences in ERK2 expression ( = 0.04). Results show antigiardial activity of kramecyne; however the mode of action is still unclear and the evaluation of ultrastructural damage and expressed proteins is an alternative of study to understand the action mechanism.
多种药物用于贾第虫病的治疗，但其效率、副作用情况以及耐药菌株的形成程度各不相同，因此寻找高效且副作用小的新型单剂量治疗方法很重要。克拉美星是从[植物名称]甲醇提取物中分离出的一种抗炎化合物，即使剂量达到5000毫克/千克也不会产生毒性。目的是确定克拉美星对[两种贾第虫名称]的抗贾第虫作用，并通过实时聚合酶链反应（RTPCR）分析克拉美星处理的滋养体中ERK1、ERK2和AK基因的表达。半数致死剂量（LD）为40微克/毫升，用台盼蓝染色和光学显微镜观察未发现形态变化；用320微克/千克体重的剂量可消除实验性沙鼠感染。治疗后，治疗组和未治疗组沙鼠的肠道之间没有差异。克拉美星对ERK1和AK没有显著影响，但ERK2表达存在差异（P = 0.04）。结果显示克拉美星具有抗贾第虫活性；然而其作用方式仍不清楚，对超微结构损伤和表达蛋白的评估是了解作用机制的一种替代研究方法。
