Department of Biology, Faculty of Science, Princess Nourah bint Abdulrahman University, Saudi Arabia.
Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt.
Biosci Rep. 2021 Feb 26;41(2). doi: 10.1042/BSR20204114.
Giardiasis is one of the most prevalent gastrointestinal diseases in the world. It is caused by Giardia, Giardia lamblia, a common and opportunistic zoonotic parasite. The aim of our work is to find a natural and safe alternative treatment for giardiasis, specifically, to determine if probiotic bacteria (Lactobacillus acidophilus, Bifidobacterium bifidum, and Lactobacillus helveticus) can contribute to treatment, and act as preventives. Sixty weanling albino mice, Mus musculus, were divided into control and experimental, probiotic-fed groups. We determined infection intensity, and cure and prevention rates of giardiasis through ELISA (enzyme-linked immunosorbent assay) of stool samples and histopathological comparison of intestinal tissue. In experimental groups, there was a significant reduction in infection intensity (P<0.001) on days 10, 15, and 20, while cure rate reached 87.5%. The control group showed no signs of reduced infection or cure and only the group treated with probiotics prior to infection showed significant prevention rates. In the experimental groups, intestinal changes due to giardiasis appeared 7 days post-infection. However, almost all of these changes disappeared by the 25th day. Our results suggest a beneficial and significant effect of probiotics in the prevention and treatment of giardiasis in mice.
贾第虫病是世界上最常见的胃肠道疾病之一。它是由贾第虫属、蓝氏贾第鞭毛虫引起的，是一种常见的机会性动物源寄生虫。我们的工作旨在寻找一种天然且安全的贾第虫病替代治疗方法，具体来说，就是确定益生菌（嗜酸乳杆菌、双歧双歧杆菌和瑞士乳杆菌）是否有助于治疗并起到预防作用。我们将 60 只断奶白化病小鼠，Mus musculus，分为对照组和实验性益生菌喂养组。我们通过粪便样本的 ELISA（酶联免疫吸附试验）和肠道组织的组织病理学比较来确定贾第虫病的感染强度、治愈率和预防率。在实验组中，第 10、15 和 20 天的感染强度显著降低（P<0.001），而治愈率达到 87.5%。对照组没有显示出感染减少或治愈的迹象，只有在感染前用益生菌治疗的组显示出显著的预防率。在实验组中，感染后 7 天出现了由贾第虫病引起的肠道变化。然而，到第 25 天，几乎所有这些变化都消失了。我们的结果表明益生菌对预防和治疗小鼠的贾第虫病具有有益且显著的效果。
