Park Jong Kang, Rowlands Christopher J, So Peter T C
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Department of Chemical Engineering and Biotechnology, Cambridge University, Cambridge CB2 1TN, UK.
Micromachines (Basel). 2017;8(3). doi: 10.3390/mi8030085. Epub 2017 Mar 9.
Temporal focusing multiphoton microscopy is a technique for performing highly parallelized multiphoton microscopy while still maintaining depth discrimination. While the conventional wide-field configuration for temporal focusing suffers from sub-optimal axial resolution, line scanning temporal focusing, implemented here using a digital micromirror device (DMD), can provide substantial improvement. The DMD-based line scanning temporal focusing technique dynamically trades off the degree of parallelization, and hence imaging speed, for axial resolution, allowing performance parameters to be adapted to the experimental requirements. We demonstrate this new instrument in calibration specimens and in biological specimens, including a mouse kidney slice.
时间聚焦多光子显微镜是一种用于执行高度并行化多光子显微镜同时仍保持深度分辨能力的技术。虽然用于时间聚焦的传统宽场配置存在轴向分辨率次优的问题，但此处使用数字微镜器件（DMD）实现的线扫描时间聚焦可以带来显著改善。基于DMD的线扫描时间聚焦技术动态地在并行化程度（进而成像速度）与轴向分辨率之间进行权衡，从而使性能参数能够适应实验要求。我们在校准标本和生物标本（包括小鼠肾脏切片）中展示了这种新仪器。
