Žurauskas Mantas, Barnstedt Oliver, Frade-Rodriguez Maria, Waddell Scott, Booth Martin J
Centre for Neural Circuits and Behaviour, University of Oxford, Mansfield Road, Oxford OX1 3SR, UK.
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK.
Biomed Opt Express. 2017 Sep 7;8(10):4369-4379. doi: 10.1364/BOE.8.004369. eCollection 2017 Oct 1.
The ability to record neural activity in the brain of a living organism at cellular resolution is of great importance for defining the neural circuit mechanisms that direct behavior. Here we present an adaptive two-photon microscope optimized for extraction of neural signals over volumes in intact brains, even in the presence of specimen motion. High speed volume imaging was made possible through reduction of spatial resolution while maintaining the light collection efficiency of a high resolution, high numerical aperture microscope. This enabled simultaneous recording of odor-evoked calcium transients in a defined volume of mushroom body Kenyon cell bodies in a live fruit fly.
在细胞分辨率下记录活生物体大脑中的神经活动，对于确定指导行为的神经回路机制至关重要。在此，我们展示了一种自适应双光子显微镜，该显微镜经过优化，可在完整大脑的体积范围内提取神经信号，即使存在样本运动也不受影响。通过降低空间分辨率同时保持高分辨率、高数值孔径显微镜的光收集效率，实现了高速体积成像。这使得在活果蝇中，能够同时记录限定体积的蘑菇体肯扬细胞体中气味诱发的钙瞬变。
