生育医学中作为天然精子抑制剂的百里醌：一项实验研究。
Thymoquinone as a natural spermostatic substance in reproductive medicine: An experimental study.
作者信息
Golshan Iranpour Farhad, Fazelian Khatereh, Dashti Gholam Reza
机构信息
Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
出版信息
Int J Reprod Biomed. 2017 Oct;15(10):641-648.
BACKGROUND
Nonoxynol-9 a nonionic surfactant is widely used for its spermicidal effects. Finding new sperm immobilizing agents is necessary because Nonoxynol-9 damages the tissues of female reproductive system.
OBJECTIVE
The aim of this study was to evaluate the effects of Thymoquinone (TQ) as a potential spermostatic compound on the motility and viability of human spermatozoa.
MATERIALS AND METHODS
In this experimental study, the effects of 5, 10, 20, 50, 100 µg/ml, 1 and 10 mg/ml of TQ on normozoospermic semen samples were investigated. Sperm motility and viability were compared between untreated and TQ-treated aliquots of each semen sample. To evaluate the effects of TQ on the alteration of mitochondrial membrane potential (MMP), 32 semen samples were examined using 50 µg/ml of TQ. Flow cytometric analysis was performed after staining of spermatozoa with JC-1.
RESULTS
Doses above 20 µg/ml of TQ could eventually immobilize all spermatozoa in culture medium. Adding 50 µg/ml of TQ did not significantly diminish the percentage of viable spermatozoa and flow cytometry results revealed that this amount of TQ could decrease sperm MMP.
CONCLUSION
TQ could discontinue the movement of sperm cells in medium without reducing the population of live spermatozoa. It is more likely that TQ exerts its spermostatic action by mitigating the MMP of spermatozoa. Therefore, TQ could be considered as a potential new natural spermostatic chemical.
背景
壬苯醇醚 -9 是一种非离子表面活性剂，因其杀精作用而被广泛使用。由于壬苯醇醚 -9 会损害女性生殖系统组织，因此寻找新的精子固定剂很有必要。
目的
本研究旨在评估百里醌（TQ）作为一种潜在的精子静止化合物对人类精子活力和生存能力的影响。
材料与方法
在本实验研究中，研究了 5、10、20、50、100 µg/ml、1 和 10 mg/ml 的 TQ 对正常精子精液样本的影响。比较了每个精液样本未经处理和经 TQ 处理的等分试样之间的精子活力和生存能力。为了评估 TQ 对线粒体膜电位（MMP）改变的影响，使用 50 µg/ml 的 TQ 检测了 32 个精液样本。用 JC -1 对精子进行染色后进行流式细胞术分析。
结果
高于 20 µg/ml 的 TQ 剂量最终可使培养基中的所有精子固定。添加 50 µg/ml 的 TQ 并未显著降低活精子的百分比，流式细胞术结果显示该剂量的 TQ 可降低精子 MMP。
结论
TQ 可使培养基中的精子细胞停止运动，而不减少活精子数量。TQ 更有可能通过降低精子的 MMP 发挥其精子静止作用。因此，TQ 可被视为一种潜在的新型天然精子静止化学物质。
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