用野豌豆或链球菌抗原分离的抗原结合性T细胞的比较研究及HLA II类抗原的作用

Comparative study of antigen binding T cells separated by Vicia villosa or streptococcal antigen and the effect of HLA class II antigens.

作者信息

Lehner T, Jones T, Avery J

出版信息

Clin Exp Immunol. 1986 Feb;63(2):312-20.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1577385/

Abstract

The antigen binding capacity and function of T cells which adhere to the lectin Vicia villosa (VV) or to streptococcal antigen (SA) have been studied. VV-adherent T8+ cells (T8+ VV+) bind 125I-SA whereas VV non-adherent T8+ cells (T8+ VV-) bind little SA. Similarly, SA-adherent T8+ cells (T8+ SA+) bind 125I-SA specifically, whereas SA-non-adherent T8+ cells (T8+ SA-) show little binding of 125I-SA. The SA binding T8+ VV+ or T8+ SA+ cells can present the antigen to T4+ helper cells which generate helper factors and these enhance anti-DNP-antibodies, when incubated with mouse spleen cells and DNP-SA. Parallel reconstitution studies with either T8+ VV- or T8+ SA- cells have revealed that both subpopulations of cells can suppress T4+ helper cell activity. Further reconstitution experiments between the T8+ VV+ (or T8+ SA+) antigen presenting cells and T8+ VV- (or T8+ SA-) suppressor cells suggest that the former can also prevent the latter cells from inhibiting T4 helper cells, so as to function as contrasuppressor cells. Cross-over, reconstitution studies between the VV and SA separated cells have confirmed that the T8+ VV+ and T8+ SA+ cells have similar functions, in SA binding, presenting and contrasuppressor activities. The dose-response curve of binding 125I-SA to T8+ cells is dependent on the HLA-DR type of cells and the binding of 125I-tetanus toxoid was similar to that of 125I-SA. Whereas DRw6- T cells bind predominantly 1,000 ng SA or tetanus toxoid, DRw6+ T cells bind 1 ng and to a lesser extent 1,000 ng of either antigen. HLA-DRw6+ subjects can be considered as high responders for their T cells bind optimally at low concentrations of antigens which induce helper and contrasuppressor functions. In contrast, HLA-DRw6-subjects can be considered as low responders, as their T cells bind optimally at high concentrations of antigens and only the latter induce helper and contrasuppressor functions.

摘要

对黏附于凝集素野豌豆（VV）或链球菌抗原（SA）的T细胞的抗原结合能力及功能进行了研究。黏附于VV的T8⁺细胞（T8⁺ VV⁺）能结合¹²⁵I-SA，而不黏附于VV的T8⁺细胞（T8⁺ VV⁻）几乎不结合SA。同样，黏附于SA的T8⁺细胞（T8⁺ SA⁺）能特异性结合¹²⁵I-SA，而不黏附于SA的T8⁺细胞（T8⁺ SA⁻）对¹²⁵I-SA的结合很少。能结合SA的T8⁺ VV⁺或T8⁺ SA⁺细胞可将抗原呈递给能产生辅助因子的T4⁺辅助细胞，当与小鼠脾细胞及DNP-SA一起孵育时，这些辅助因子可增强抗DNP抗体的产生。用T8⁺ VV⁻或T8⁺ SA⁻细胞进行的平行重建研究表明，这两个细胞亚群均能抑制T4⁺辅助细胞的活性。T8⁺ VV⁺（或T8⁺ SA⁺）抗原呈递细胞与T8⁺ VV⁻（或T8⁺ SA⁻）抑制细胞之间的进一步重建实验表明，前者还可阻止后者抑制T4辅助细胞，从而起到抗抑制细胞的作用。对VV和SA分离细胞进行的交叉重建研究证实，T8⁺ VV⁺和T8⁺ SA⁺细胞在SA结合能力、呈递能力及抗抑制活性方面具有相似的功能。¹²⁵I-SA与T8⁺细胞结合的剂量反应曲线取决于细胞的HLA-DR类型，¹²⁵I-破伤风类毒素的结合情况与¹²⁵I-SA相似。DRw6⁻ T细胞主要结合1000 ng SA或破伤风类毒素，而DRw6⁺ T细胞结合1 ng，且对1000 ng两种抗原的结合程度较低。HLA-DRw6⁺受试者可被视为高反应者，因为其T细胞在低浓度抗原下能最佳结合，从而诱导辅助和抗抑制功能。相比之下，HLA-DRw6⁻受试者可被视为低反应者，因为其T细胞在高浓度抗原下能最佳结合，且只有高浓度抗原才能诱导辅助和抗抑制功能。