Green D R, Gold J, St Martin S, Gershon R, Gershon R K
Proc Natl Acad Sci U S A. 1982 Feb;79(3):889-92. doi: 10.1073/pnas.79.3.889.
The addition of Peyer's patch T cells from most strains of mice to spleen cells in primary Mishell-Dutton cultures either has no effect or augments the spleen cells' response to sheep erythrocytes. However, if the Peyer's patch T cells are treated with an anti-I-J antiserum and complement to remove contrasuppressor-inducer cells, the remaining Ly-2 cells (T cells that express Ly-2 but not Ly-1) are highly suppressive. This "latent" suppressor cell activity also can be revealed by removing contrasuppressor-acceptor (transducer) cells from the splenic assay population with either an anti-I-J or anti-Ly-2 antiserum. These findings, taken together with previous work showing that orally administered antigen leads to systemic tolerance, give experimental support to the notion that contrasuppression may be important in allowing microenvironmental immune responses (in this case the gut-associated lymphoid tissue) to take place while systemic immunity is suppressed.
在初次米舍尔-达顿培养中，将大多数品系小鼠的派尔集合淋巴结T细胞添加到脾细胞中，要么没有效果，要么增强脾细胞对绵羊红细胞的反应。然而，如果用抗I-J抗血清和补体处理派尔集合淋巴结T细胞以去除抗抑制诱导细胞，剩余的Ly-2细胞（表达Ly-2但不表达Ly-1的T细胞）具有高度抑制性。通过用抗I-J或抗Ly-2抗血清从脾检测群体中去除抗抑制受体（转导细胞），也可以揭示这种“潜在”的抑制细胞活性。这些发现，连同先前表明口服抗原导致全身耐受的研究工作，为抗抑制在全身免疫受到抑制时允许微环境免疫反应（在这种情况下是肠道相关淋巴组织）发生中可能起重要作用的观点提供了实验支持。
