Conzelmann A, Pink R, Acuto O, Mach J P, Dolivo S, Nabholz M
Eur J Immunol. 1980 Nov;10(11):860-8. doi: 10.1002/eji.1830101111.
Murine cytolytic T cell lines have been analyzed for the expression of two surface glycoproteins called T145 and T130. T145, known to be expressed by activated cytolytic T cells, is also expressed by such lines, but T130, which has been described by a universal T cell marker, is not. Our results suggest a structural relationship between T145 and T130. Vicia villosa lectin, which binds selectively to T145 of activated T cells and which is cytotoxic for cytolytic T cell lines, has been used to select lectin-resistant mutants from these lines. Five independent lectin-resistant mutants have been obtained. All of them are cytolytically active, bind up to 100-fold less lectin than the parental lines, but still express T145 or a closely related glycoprotein.
对小鼠细胞溶解T细胞系进行了分析，以检测两种表面糖蛋白T145和T130的表达情况。已知T145由活化的细胞溶解T细胞表达，这些细胞系也表达T145，但一种通用T细胞标志物所描述的T130却不表达。我们的结果表明T145和T130之间存在结构关系。野豌豆凝集素可选择性结合活化T细胞的T145，且对细胞溶解T细胞系具有细胞毒性，已被用于从这些细胞系中筛选凝集素抗性突变体。已获得五个独立的凝集素抗性突变体。它们都具有细胞溶解活性，与亲代细胞系相比，结合的凝集素少至100倍，但仍表达T145或一种密切相关的糖蛋白。
