Dalmastri Claudia, Gastaldo Luciano, Marcone Giorgia Letizia, Binda Elisa, Congiu Terenzio, Marinelli Flavia
ENEA (Italian National Agency for New Technologies, Energy and Sustainable Economic Development), Department for Sustainability of Production and Territorial Systems, via Anguillarese 301, 00123, Rome, Italy.
Department of Biotechnology and Life Sciences, University of Insubria, and 'The Protein Factory' Research Center, Politecnico of Milano, ICRM CNR Milano and University of Insubria, 21100, Varese, Italy.
Int J Syst Evol Microbiol. 2016 Feb;66(2):912-921. doi: 10.1099/ijsem.0.000810.
Strain ATCC 39727, which produces the antibiotic A40926 (the natural precursor of the antibiotic dalbavancin), was isolated from a soil sample collected in India, and it was originally classified as a member of the genus Actinomadura on the base of morphology and cell-wall composition. A phylogenetic analysis based on 16S rRNA gene sequences indicates that the strain forms a distinct clade within the genus Nonomuraea, and it is most closely related to Nonomuraea angiospora DSM 43173T (98.72 % similarity) and Nonomuraea jabiensis A4036T (98.69 %). The strain forms an extensively branched substrate mycelium and aerial hyphae that form spiral chains of spores with ridged surfaces. The cell wall contains meso-diaminopimelic acid and the whole-cell sugars are glucose, ribose, galactose, mannose and madurose (madurose as the diagnostic sugar). The N-acyl type of muramic acid is acetyl. The predominant menaquinone is MK-9(H4), with minor amounts of MK-9(H2), MK-9(H6) and MK-9(H0). The polar-lipid profile includes diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylmethylethanolamine, phosphatidylinositol and a series of uncharacterized phospholipids, glycolipids and phosphoglycolipids. The major cellular fatty acids are iso-C16 : 0 and 10-methyl C17 : 0. The genomic DNA G+C content is 71.2 mol%. Significant differences in the morphological, chemotaxonomic and biochemical data, together with DNA-DNA relatedness between strain ATCC 39727 and closely related type strains, clearly demonstrated that strain ATCC 39727 represents a novel species of the genus Nonomuraea, for which the name Nonomuraea gerenzanensis sp. nov. is proposed. The type strain is ATCC 39727T ( = DSM 100948T).
菌株ATCC 39727可产生抗生素A40926（达巴万星的天然前体），它是从印度采集的一份土壤样本中分离得到的，最初根据形态学和细胞壁组成被归类为马杜拉放线菌属的一个成员。基于16S rRNA基因序列的系统发育分析表明，该菌株在野野村菌属内形成一个独特的分支，与野野村菌DSM 43173T（相似性98.72%）和贾比野野村菌A4036T（相似性98.69%）关系最为密切。该菌株形成广泛分支的基内菌丝和气生菌丝，气生菌丝形成表面有脊的螺旋状孢子链。细胞壁含有内消旋二氨基庚二酸，全细胞糖为葡萄糖、核糖、半乳糖、甘露糖和马杜拉糖（马杜拉糖为鉴别性糖）。胞壁酸的N-酰基类型为乙酰基。主要的甲基萘醌是MK-9(H4)，还有少量的MK-9(H2)、MK-9(H6)和MK-9(H0)。极性脂谱包括双磷脂酰甘油、磷脂酰乙醇胺、羟基磷脂酰乙醇胺、磷脂酰甲基乙醇胺、羟基磷脂酰甲基乙醇胺、磷脂酰肌醇以及一系列未鉴定的磷脂、糖脂和磷酸糖脂。主要的细胞脂肪酸是异-C16 : 0和10-甲基-C17 : 0。基因组DNA的G+C含量为71.2mol%。菌株ATCC 39727与密切相关的模式菌株在形态、化学分类和生化数据以及DNA-DNA相关性方面存在显著差异，这清楚地表明菌株ATCC 39727代表野野村菌属的一个新物种，为此提出新名称格伦赞野野村菌（Nonomuraea gerenzanensis sp. nov.）。模式菌株为ATCC 39727T（=DSM 100948T）。
