Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan.
Department of Industrial Engineering and Systems Management, Feng-Chia University, Taichung, Taiwan.
J Food Drug Anal. 2018 Jan;26(1):182-192. doi: 10.1016/j.jfda.2017.02.002. Epub 2017 Mar 16.
This study aimed to investigate the antioxidant and anticancer effects of ethanolic and aqueous extracts of the roots of Ficus beecheyana (EERFB and AERFB) and their phenolic components. In this study, total phenolic content and antioxidant activity of EERFB were higher than those of AERFB. Major phenolic compounds in the extracts were gallic acid, p-hydroxybenzoic acid, caffeic acid, chlorogenic acid, p-coumaric acid, and rutin; which were identified by high-performance liquid chromatography. Flow cytometric analysis of HL-60 cells exposed to EERFB showed that the percentage of apoptotic cells increased in a dose-dependent manner. EERFB treatment resulted in the loss of mitochondrial membrane potential and induced the apoptosis of HL-60 cells through a Fas- and mitochondrial-mediated pathway. Finally, pretreatment with general caspase-9/-3 inhibitors prevented EERFB from inhibiting cell viability in HL-60 cells. Our finding suggests that EERFB is an agent that may have antioxidant activity and inhibit the growth of cancer cells.
本研究旨在探讨榕属植物根的乙醇和水提取物（EERFB 和 AERFB）及其酚类成分的抗氧化和抗癌作用。在这项研究中，EERFB 的总酚含量和抗氧化活性均高于 AERFB。提取物中的主要酚类化合物为没食子酸、对羟基苯甲酸、咖啡酸、绿原酸、对香豆酸和芦丁；这些化合物通过高效液相色谱法得到鉴定。用流式细胞术分析暴露于 EERFB 的 HL-60 细胞显示，细胞凋亡的百分比呈剂量依赖性增加。EERFB 处理导致线粒体膜电位丧失，并通过 Fas 和线粒体介导的途径诱导 HL-60 细胞凋亡。最后，用一般的 caspase-9/-3 抑制剂预处理可防止 EERFB 抑制 HL-60 细胞的细胞活力。我们的研究结果表明，EERFB 是一种具有抗氧化活性和抑制癌细胞生长的物质。
