Nunes Ana R, Gonçalves Ana C, Alves Gilberto, Falcão Amílcar, Garcia-Viguera Cristina, A Moreno Diego, Silva Luís R
CICS-UBI-Health Sciences Research Center, University of Beira Interior, 6200-506 Covilhã, Portugal.
CNC-Centre for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal.
Foods. 2021 May 25;10(6):1185. doi: 10.3390/foods10061185.
L. by-products, such as stems, leaves, and flowers, are used in folk medicine to prevent and treat some diseases. However, their phenolic composition and in vitro bioactivities against tumor cells are poorly known. In this work, we compared the phenolic profile and the biological potential of aqueous infusions and hydroethanolic extracts of leaves, stems, and flowers from Saco cultivar, collected from the Fundão region (Portugal). Among the fifty-two phenolic compounds tentatively identified by HPLC-DAD-ESI/MS, the hydroxycinnamic acids were the most abundant. Both extracts of stems revealed a higher activity against DPPH. Meanwhile, hydroethanolic extracts from stems and flowers and aqueous infusions of flowers were the most effective in inhibiting the growth of the human epithelial colorectal adenocarcinoma (Caco-2) cells at concentrations above 200 μg/mL. More detailed knowledge about the phenolic composition and health-promoting properties of Portuguese by-products allows for increasing the biological and commercial value of these bio-wastes, which may have a positive impact on food and pharmaceutical industries, as on the valorization of the local economy.
L. 的副产品，如茎、叶和花，在民间医学中用于预防和治疗某些疾病。然而，它们的酚类成分以及对肿瘤细胞的体外生物活性却鲜为人知。在这项工作中，我们比较了从葡萄牙丰当地区采集的萨科品种的叶、茎和花的水浸液和乙醇提取物的酚类特征和生物潜力。在通过HPLC-DAD-ESI/MS初步鉴定的52种酚类化合物中，羟基肉桂酸最为丰富。两种茎提取物对DPPH显示出更高的活性。同时，茎和花的乙醇提取物以及花的水浸液在浓度高于200μg/mL时对人上皮结直肠癌（Caco-2）细胞的生长抑制最为有效。对葡萄牙副产品的酚类成分和健康促进特性有更详细的了解，可以提高这些生物废料的生物和商业价值，这可能对食品和制药行业以及当地经济的增值产生积极影响。
