搏动流灌注对脱细胞化的影响。
Effect of pulsatile flow perfusion on decellularization.
机构信息
School of Medicine, Kangwon National University, Chuncheon-si, Republic of Korea.
Program of Mechanical and Biomedical Engineering, College of Engineering, Kangwon National University, Chuncheon-si, Republic of Korea.
出版信息
Biomed Eng Online. 2018 Feb 1;17(1):15. doi: 10.1186/s12938-018-0445-0.
BACKGROUND
Decellularized animal organs have been used as scaffolds for tissue engineering. To make a properly functioning scaffolds, the extracellular matrix (ECM) components must be preserved after decellularization. Because pulsatile flow is known to be beneficial for tissue perfusion, pulsatile perfusion of a detergent might decrease the exposure time of the tissues to the detergent used for decellularization. Using Energy Equivalent Pressure (EEP) as a pulsatility parameter, the effect of pulsatile flow in decellularization process is studied.
RESULTS
Twelve rat hearts were decellularization with 1% sodium dodecyl sulfate (SDS) solution for 2 h. They are divided into two groups, one with pulsatile perfusion (n = 6), the other with non-pulsatile perfusion (n = 6) of SDS. The initial mean perfusion pressures were same in both group. The result indicated that the EEP and the perfusion flow were increased significantly in the pulsatile group compared to the non-pulsatile group. Photographs taken during the decellularization showed more profound decellularization in the pulsatile group. The residual DNA content in the scaffolds was significantly lower in the pulsatile group. However, the level of ECM components, collagen and GAG showed no significant differences between the groups.
CONCLUSIONS
Decellularization is more efficient in pulsatile flow than in non-pulsatile flow but still preserves the ECM molecules.
背景
脱细胞动物器官已被用作组织工程的支架。为了制造出功能正常的支架，脱细胞后必须保留细胞外基质 (ECM) 成分。由于脉动流已知对组织灌注有益，因此脉动灌注去污剂可能会减少组织暴露于用于脱细胞的去污剂的时间。使用能量等效压力 (EEP) 作为脉动参数，研究了脉动流在脱细胞过程中的作用。
结果
用 1%十二烷基硫酸钠 (SDS) 溶液对 12 个大鼠心脏进行脱细胞处理 2 小时。它们分为两组，一组进行脉动灌注 (n=6)，另一组进行 SDS 非脉动灌注 (n=6)。两组的初始平均灌注压力相同。结果表明，与非脉动组相比，脉动组的 EEP 和灌注流量显着增加。脱细胞过程中拍摄的照片显示脉动组的脱细胞程度更深。支架中的残留 DNA 含量在脉动组中显着降低。然而，两组之间的 ECM 成分（胶原和 GAG）水平没有显着差异。
结论
与非脉动流相比，脉动流可更有效地进行脱细胞，但仍能保留 ECM 分子。
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