一种用于检测玉米赤霉醇及其代谢物的化学发光免疫分析法。
A chemiluminescent immunoassay for zeranol and its metabolites.
作者信息
Jansen E H, van den Berg R H, Zomer G, Enkelaar-Willemsen C, Stephany R W
出版信息
J Vet Pharmacol Ther. 1986 Mar;9(1):101-8. doi: 10.1111/j.1365-2885.1986.tb00018.x.
A sensitive immunoassay is presented for the detection of the xenobiotic oestrogenic growth promotor zeranol (Ralgro) and the mycotoxin zearalanone. The detection is based on chemiluminescence using a zeranol-isoluminol conjugate. A lowest detectable amount of 2-5 pg zeranol per tube was achieved. The bovine zeranol metabolites zearalanone and taleranol can also be determined, and this has been shown in a metabolic pilot study of zeranol in veal calves. This study indicates that taleranol is a major metabolite in male veal calves, in contrast to zearalanone which is known to be the major metabolite in adult cattle.
本文介绍了一种灵敏的免疫分析法，用于检测外源性雌激素生长促进剂玉米赤霉醇（Ralgro）和霉菌毒素玉米赤霉烯酮。该检测基于使用玉米赤霉醇-异鲁米诺共轭物的化学发光法。每管最低可检测到2-5皮克玉米赤霉醇。还可以测定牛体内玉米赤霉醇的代谢产物玉米赤霉烯酮和塔勒醇，这已在犊牛玉米赤霉醇的代谢初步研究中得到证实。该研究表明，与已知是成年牛主要代谢产物的玉米赤霉烯酮相比，塔勒醇是雄性犊牛的主要代谢产物。
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