Department of Urology, The Fifth People's Hospital of Jinan, Jinan, Shandong 250012, P.R. China.
Tianqiao Hospital in Jinan of Shandong, Jinan, Shandong 250022, P.R. China.
Int J Mol Med. 2018 Apr;41(4):2429-2433. doi: 10.3892/ijmm.2018.3421. Epub 2018 Jan 23.
Previous literature exists on the role of microRNA (miR)-132 in initiation and progression of various malignancies. In this study, we aimed at understanding the relationship of miR-132 of prostate tumorigenesis. We collected 32 prostate cancer tissues and adjacent non-cancerous controls, and detected the expression level of miR-132. Then the miRNA database was searched online and luciferase assay perform to understand the regulatory relationship between miR-132 and E2F5. Moreover, we also conducted real-time PCR and western blot analysis to study the mRNA and protein expression level of E2F5 among different groups (cancerous tissue, n=32; non-cancerous tissue, n=32) or cells treated with scramble control, miR-132 mimics, E2F5 siRNA and miR-132 inhibitors. miR-132 was upregulated in cancerous tissues of prostate cancer patients. E2F5 was the target of miR-132, and negative regulatory relationship between miR-132 and E2F5 was also confirmed by luciferase assay. The mRNA and protein expression level of E2F5 increased in cancerous tissue group. miR-132 decreased the expression of E2F5 in prostate cancer cells, and introduction of miR-132 reduced the viability and E2F5 and promoted the viability of prostate cancer cells. miR-132 inhibited apoptosis and E2F5 accelerated apoptosis. In conclusion, miR-132 was upregulated in cancerous tissue of prostate cancer. E2F5 was a direct target of miR-132, and downregulation of E2F5 caused by upregulation of miR-132 may contribute to the tumorigenesis of prostate cancer.
先前的文献研究了 microRNA（miR）-132 在各种恶性肿瘤的发生和发展中的作用。在本研究中，我们旨在了解 miR-132 与前列腺肿瘤发生之间的关系。我们收集了 32 例前列腺癌组织和相邻的非癌对照组织，检测了 miR-132 的表达水平。然后在网上搜索 miRNA 数据库并进行荧光素酶检测，以了解 miR-132 与 E2F5 之间的调控关系。此外，我们还通过实时 PCR 和 Western blot 分析研究了不同组（癌组织，n=32；非癌组织，n=32）或用 scramble 对照、miR-132 模拟物、E2F5 siRNA 和 miR-132 抑制剂处理的细胞中 E2F5 的 mRNA 和蛋白表达水平。miR-132 在前列腺癌患者的癌组织中上调。E2F5 是 miR-132 的靶基因，荧光素酶检测也证实了 miR-132 与 E2F5 之间存在负向调控关系。癌组织组中 E2F5 的 mRNA 和蛋白表达水平增加。miR-132 降低前列腺癌细胞中 E2F5 的表达，引入 miR-132 降低了前列腺癌细胞的活力并促进了其活力。miR-132 抑制细胞凋亡，而 E2F5 加速细胞凋亡。总之，miR-132 在前列腺癌癌组织中上调。E2F5 是 miR-132 的直接靶基因，miR-132 上调导致 E2F5 下调可能有助于前列腺癌的发生。
