Domingos G M, Baldin E L L, Canassa V F, Silva I F, Lourenção A L
Depto de Proteção Vegetal, Fac de Ciências Agronômicas, Univ Estadual Paulista (UNESP), Botucatu, SP, 18610-307, Brasil.
Instituto Agronômico, Centro de Fitossanidade, Campinas, SP, Brasil.
Neotrop Entomol. 2018 Aug;47(4):560-568. doi: 10.1007/s13744-018-0588-5. Epub 2018 Feb 2.
Bemisia tabaci (Genn.) biotype B (Hemiptera: Aleyrodidae) is an important pest of vegetable crops, including collard greens Brassica oleracea var. acephala (Brassicaceae). The use of resistant genotypes is an interesting option to reduce insect populations and can be used as an important tool for integrated pest management (IPM). This study evaluated 32 genotypes of collard greens against the attack of silver leaf whitefly, with the aim to characterize antixenosis. Initially, a multiple-choice trial was conducted using all genotypes, in which the adult attractiveness was assessed on two leaves per genotype at 24 and 48 h after infestation. After 48 h, one leaf of each genotype was randomly selected for the determination of the number of eggs per square centimeter. From the results of the multiple-choice trial, 13 genotypes were selected for a no-choice oviposition test, following the same method of the previous test. Colorimetric analyses were also performed to establish possible correlations between leaf color and insect colonization. Genotypes HS-20, OE, and VA were less attractive, demonstrating antixenosis. Genotypes LG, VE, J, MG, MOP, HS-20, VA, and MT had less oviposition in the multiple-choice test, which indicated expression of antixenosis. In the no-choice test, genotypes VE, P1C, CCB, RI-919, H, and J had less oviposition, which also characterized antixenosis. Therefore, genotypes VE and J showed the highest resistance stability because both had less oviposition in both test modalities. Thus, the resistance to B. tabaci biotype B indicates the genotypes HS-20, OE, VA, VE, and J are promising for use in breeding programs to develop resistance to whitefly.
烟粉虱（Bemisia tabaci (Genn.)）生物型B（半翅目：粉虱科）是包括羽衣甘蓝（Brassica oleracea var. acephala，十字花科）在内的蔬菜作物的重要害虫。使用抗性基因型是减少昆虫种群数量的一个有吸引力的选择，并且可作为害虫综合治理（IPM）的重要工具。本研究评估了32个羽衣甘蓝基因型对银叶粉虱侵害的抗性，旨在鉴定抗生性。最初，使用所有基因型进行了多项选择试验，在侵染后24小时和48小时评估每个基因型两片叶子上成虫的趋性。48小时后，随机选择每个基因型的一片叶子，测定每平方厘米的卵数。根据多项选择试验的结果，按照前一个试验的相同方法，选择13个基因型进行非选择产卵试验。还进行了比色分析，以确定叶色与昆虫定殖之间可能的相关性。基因型HS-20、OE和VA的吸引力较小，表现出抗生性。基因型LG、VE、J、MG、MOP、HS-20、VA和MT在多项选择试验中的产卵量较少，这表明存在抗生性。在非选择试验中，基因型VE、P1C、CCB、RI-919、H和J的产卵量较少，这也表现出抗生性。因此，基因型VE和J表现出最高的抗性稳定性，因为它们在两种试验模式下的产卵量都较少。因此，对烟粉虱生物型B的抗性表明，基因型HS-20、OE、VA、VE和J有望用于育种计划，以培育对白粉虱具有抗性的品种。
