Yakult Central Institute, 5-11 Izumi, Kunitachi-shi, Tokyo 186-8650, Japan.
Yakult Central Institute, 5-11 Izumi, Kunitachi-shi, Tokyo 186-8650, Japan.
J Dairy Sci. 2018 Apr;101(4):2838-2841. doi: 10.3168/jds.2017-13868. Epub 2018 Feb 4.
Probiotic lactobacilli stimulate macrophages and dendritic cells to secrete cytokines and thereby regulate the immune responses of the host. The balance of the IL-10 and IL-12 production induced by a probiotic is crucial for determining the direction of the immune response. In the present study, we examined the ability of microbial components to modify IL-10 and IL-12 production induced by a popular probiotic strain, Lactobacillus casei strain Shirota (LcS), which itself predominantly induces IL-12 production. Microbial ligands for toll-like receptor (TLR)3 and TLR5 further enhanced the IL-12 induction by LcS, whereas ligands for TLR2, TLR4, TLR7, and TLR9 converted the cytokine production pattern from IL-12 predominant to IL-10 predominant. These results indicate that the probiotic induction of IL-10 and IL-12 production can be flexibly modified by co-stimulation with microbial components. This could explain the variety of immunomodulatory functions (immunoactivation or anti-inflammation) exerted by this probiotic strain.
益生菌乳杆菌刺激巨噬细胞和树突状细胞分泌细胞因子，从而调节宿主的免疫反应。益生菌诱导的 IL-10 和 IL-12 产生的平衡对于决定免疫反应的方向至关重要。在本研究中，我们研究了微生物成分改变由广受欢迎的益生菌菌株干酪乳杆菌 Shirota（LcS）诱导的 IL-10 和 IL-12 产生的能力，LcS 主要诱导 IL-12 的产生。TLR3 和 TLR5 的微生物配体进一步增强了 LcS 的 IL-12 诱导作用，而 TLR2、TLR4、TLR7 和 TLR9 的配体将细胞因子产生模式从以 IL-12 为主转变为以 IL-10 为主。这些结果表明，益生菌诱导的 IL-10 和 IL-12 产生可以通过与微生物成分的共刺激而灵活地改变。这可以解释该益生菌菌株发挥的各种免疫调节功能（免疫激活或抗炎）。
