Department of Microbiology, AIIMS Bhopal, Madhya Pradesh, India.
CSIR-AMPRI, Bhopal 462026, Madhya Pradesh, India.
Am J Infect Control. 2018 Jun;46(6):e37-e42. doi: 10.1016/j.ajic.2017.12.015. Epub 2018 Feb 2.
Although disinfectants are used for eradication of bacteria from environmental surfaces, their antibiofilm efficacy is often not considered in determining the choice of disinfectant.
This study aimed to compare the effectiveness of 2 commonly used disinfectants, sodium hypochlorite and ethanol, against the planktonic and biofilm state of Staphylococcus aureus clinical isolates.
Effect of 0.6% sodium hypochlorite and 70% ethanol was determined on the planktonic and biofilm states of 10 strong and weak biofilm formers through estimation of changes in colony forming unit counts and absorbance values. The morphologic changes were observed by scanning electron microscopy.
Significant difference in the efficacy of sodium hypochlorite and ethanol was observed against the biofilm (P = .004) as well as planktonic (P = .000) states of S aureus. However, no significant difference was observed in their activity against strong and weak biofilm formers. On electron microscopy, sodium hypochlorite was found to induce significant formation of craters and irregular depressions on the surface of strong biofilm formers.
Sodium hypochlorite demonstrated superior efficacy in controlling both planktonic and biofilm states of growth in S aureus. Furthermore, the characteristic morphologic changes observed in strong biofilm formers hint at its biofilm-specific activity.
尽管消毒剂用于消灭环境表面的细菌，但在选择消毒剂时，通常不考虑其抗生物膜的功效。
本研究旨在比较两种常用消毒剂次氯酸钠和乙醇对金黄色葡萄球菌临床分离株浮游和生物膜状态的有效性。
通过评估菌落形成单位计数和吸光度值的变化，确定 0.6%次氯酸钠和 70%乙醇对 10 株强和弱生物膜形成菌的浮游和生物膜状态的影响。通过扫描电子显微镜观察形态变化。
次氯酸钠和乙醇对金黄色葡萄球菌生物膜（P=0.004）和浮游状态（P=0.000）的疗效存在显著差异。然而，它们对强和弱生物膜形成菌的活性没有显著差异。电子显微镜观察发现，次氯酸钠可在强生物膜形成菌表面诱导明显的火山口和不规则凹陷形成。
次氯酸钠在控制金黄色葡萄球菌浮游和生物膜生长状态方面表现出优异的功效。此外，在强生物膜形成菌中观察到的特征形态变化提示其具有生物膜特异性活性。
