三硅酸钙基修复材料皮下植入大鼠后组织反应和白细胞介素 6 的免疫表达。

Tissue Response and Immunoexpression of Interleukin 6 Promoted by Tricalcium Silicate-based Repair Materials after Subcutaneous Implantation in Rats.

机构信息

Department of Restorative Dentistry, Araraquara Dental School, UNESP-São Paulo State University, Araraquara, São Paulo, Brazil.

Institute of Clinical Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.

出版信息

J Endod. 2018 Mar;44(3):458-463. doi: 10.1016/j.joen.2017.12.006.

DOI:10.1016/j.joen.2017.12.006

PMID:29398089

Abstract

INTRODUCTION

The aim of the present study was to evaluate the inflammatory response induced by experimental tricalcium silicate cement with 20% zirconium oxide (TSC) and MTA Plus (MTAP; Avalon Biomed Inc, Bradenton, FL) in rat subcutaneous tissues.

METHODS

Polyethylene tubes were filled with TSC (n = 20) and MTAP (n = 20) and implanted in the dorsal subcutaneous tissues of 32 rats. Empty tubes were used as the control (control group [CG], n = 20). After 7, 15, 30, and 60 days, the tubes with connective tissue were removed, and the inflammatory cells and immunolabeled cells for interleukin 6 (IL-6) were counted. Data were statistically analyzed using analysis of variance and the Tukey test (P ≤ .05).

RESULTS

An increased number of inflammatory and immunolabeled cells for IL-6 were observed at 7 days. The number of inflammatory cells was higher for TSC and MTAP than the CG (P < .001) at 7 days; after 30 and 60 days, no significant differences were observed among the TSC, MTAP, and CG (P = .955). The number of immunolabeled cells for IL-6 was similar for TSC, MTAP, and CG at all evaluated periods. A gradual and significant decrease was observed in the number of inflammatory cells and IL-6-immunopositive cells. At 60 days, the capsules adjacent to TSC and MTAP exhibited fibroblasts and bundles of collagen fibers.

CONCLUSIONS

TSC and MTAP caused a similar subcutaneous reaction in rats, suggesting that they are biocompatible and present similar immune responses.

摘要

简介

本研究旨在评估含 20%氧化锆的硅酸三钙水泥（TSC）和 MTA Plus（Avalon Biomed Inc，Bradenton，FL）在大鼠皮下组织中引起的炎症反应。

方法

将聚乙烯管填充 TSC（n=20）和 MTAP（n=20）并植入 32 只大鼠的背部皮下组织。空管用作对照（CG，n=20）。在第 7、15、30 和 60 天，取出带有结缔组织的管，并对炎症细胞和白细胞介素 6（IL-6）免疫标记细胞进行计数。使用方差分析和 Tukey 检验对数据进行统计学分析（P≤.05）。

结果

在第 7 天观察到炎症细胞和免疫标记的 IL-6 细胞数量增加。在第 7 天，TSC 和 MTAP 的炎症细胞数量高于 CG（P<.001）；在第 30 和 60 天，TSC、MTAP 和 CG 之间没有观察到显著差异（P=0.955）。在所有评估的时间段内，TSC、MTAP 和 CG 的免疫标记的 IL-6 细胞数量相似。炎症细胞和 IL-6 阳性细胞的数量逐渐减少且具有统计学意义。在第 60 天，TSC 和 MTAP 附近的胶囊表现为成纤维细胞和胶原纤维束。