Rose Max, Senkbeil Tobias, von Gundlach Andreas R, Stuhr Susan, Rumancev Christoph, Dzhigaev Dmitry, Besedin Ilya, Skopintsev Petr, Loetgering Lars, Viefhaus Jens, Rosenhahn Axel, Vartanyants Ivan A
Opt Express. 2018 Jan 22;26(2):1237-1254. doi: 10.1364/OE.26.001237.
Coherent X-ray ptychography is a tool for highly dose efficient lensless nano-imaging of biological samples. We have used partially coherent soft X-ray synchrotron radiation to obtain a quantitative image of a laterally extended, dried, and unstained fibroblast cell by ptychography. We used data with and without a beam stop that allowed us to measure coherent diffraction with a high-dynamic range of 1.7·10. As a quantitative result, we obtained the refractive index values for two regions of the cell with respect to a reference area. Due to the photon energy in the water window we obtained an extremely high contrast of 53% at 71 nm half-period resolution. The dose applied in our experiment was 9.5·10 Gy and is well below the radiation damage threshold. The concept for dynamic range improvement for low dynamic range detectors with a beam stop opens the path for high resolution nano-imaging of a variety of samples including cryo-preserved, hydrated and unstained biological cells.
相干X射线叠层成像术是一种用于对生物样品进行高剂量效率无透镜纳米成像的工具。我们利用部分相干软X射线同步辐射，通过叠层成像术获得了横向延伸、干燥且未染色的成纤维细胞的定量图像。我们使用了有无束流阻挡器的数据，这使我们能够在1.7·10的高动态范围内测量相干衍射。作为定量结果，我们获得了细胞两个区域相对于参考区域的折射率值。由于处于水窗中的光子能量，我们在71纳米半周期分辨率下获得了高达53%的极高对比度。我们实验中施加的剂量为9.5·10 Gy，远低于辐射损伤阈值。使用束流阻挡器提高低动态范围探测器动态范围的概念，为包括冷冻保存、水合且未染色的生物细胞在内的各种样品的高分辨率纳米成像开辟了道路。
