Department of Pharmacy, Faculty of Medicine, University of Nis, Nis, Serbia.
Department of Physiology, Faculty of Medicine, University of Nis, Nis, Serbia.
Med Princ Pract. 2018;27(2):179-185. doi: 10.1159/000487202. Epub 2018 Jan 28.
The purpose of this study was to investigate the relaxative effects of blackcurrant juice on the gastrointestinal smooth muscle in vitro.
Berries of the blackcurrant cultivar Ometa were used for the preparation of the juice used. The spasmolytic activity of blackcurrant juice was tested on rat ileum isolated from male Wistar rats by monitoring its influence on spontaneous contractions, as well as contractions induced by potassium chloride (KCl), barium chloride (BaCl2), calcium chloride (CaCl2), and acetylcholine (Ach). The results are expressed as the mean ± standard deviation obtained in 6 measurements and statistical significance was determined by the Student t test, with p < 0.05 taken as significant.
The blackcurrant cultivar Ometa significantly reduced the frequency and the amplitude of spontaneous contractions (57.94 ± 3.44%) and Ach-induced contractions (42.74 ± 5.36%; p < 0.05) of the isolated rat ileum. Cumulative concentrations (0.01-3 mg/mL) of the Ometa juice also reduced contractions of the isolated rat ileum stimulated by KCl (51.46 ± 6.87%), CaCl2 (57.54 ± 6.47%), and BaCl2 (58.54 ± 10.55%). The inhibitory effects of the juice were proportional to the applied concentration.
The antispasmodic effect of Ometa cultivar shows that common gastrointestinal disorders could be treated by the functional food.
本研究旨在探讨黑加仑汁对胃肠道平滑肌的舒张作用。
使用 Ometa 品种黑加仑浆果制备黑加仑汁。通过监测其对自发性收缩以及氯化钾 (KCl)、氯化钡 (BaCl2)、氯化钙 (CaCl2) 和乙酰胆碱 (Ach) 诱导收缩的影响，测试黑加仑汁的解痉活性。结果以 6 次测量得到的平均值 ± 标准差表示，并通过学生 t 检验确定统计学意义，p < 0.05 为显著差异。
Ometa 黑加仑品种显著降低了离体大鼠回肠的自发性收缩频率和幅度（57.94 ± 3.44%）和 Ach 诱导的收缩（42.74 ± 5.36%；p < 0.05）。Ometa 汁的累积浓度（0.01-3 mg/mL）还降低了 KCl（51.46 ± 6.87%）、CaCl2（57.54 ± 6.47%）和 BaCl2（58.54 ± 10.55%）刺激的离体大鼠回肠收缩。该汁的抑制作用与应用浓度成正比。
Ometa 品种的抗痉挛作用表明，功能性食品可用于治疗常见的胃肠道疾病。
