University College London, Institute for Women's Health, Maternal and Fetal Medicine Department, London, UK.
University College London, Great Ormond Street Institute for Child Health, London, UK.
Sci Rep. 2018 Feb 5;8(1):2425. doi: 10.1038/s41598-018-20710-9.
Human amniotic fluid contains two morphologically-distinct sub-populations of stem cells with regenerative potential, spindle-shaped (SS-hAFSCs) and round-shaped human amniotic fluid stem cells (RS-hAFSCs). However, it is unclear whether morphological differences correlate with functionality, and this lack of knowledge limits their translational applications. Here, we show that SS-hAFSCs and RS-hAFSCs differ in their neuro-protective ability, demonstrating that a single contralateral injection of SS-hAFSCs into hypoxic-ischemic P7 mice conferred a 47% reduction in hippocampal tissue loss and 43-45% reduction in TUNEL-positive cells in the hippocampus and striatum 48 hours after the insult, decreased microglial activation and TGFβ1 levels, and prevented demyelination. On the other hand, RS-hAFSCs failed to show such neuro-protective effects. It is possible that SS-hAFSCs exert their neuroprotection via endoglin-dependent inhibition of TGFβ1 signaling in target cells. These findings identify a sub-population of CD117CD90CD105 stem cells as a promising source for the neuro-protection of the developing brain.
人羊水含有两种具有再生潜力的形态上不同的干细胞亚群，即纺锤形（SS-hAFSCs）和圆形人羊水干细胞（RS-hAFSCs）。然而，目前尚不清楚形态差异是否与功能相关，这种知识的缺乏限制了它们的转化应用。在这里，我们表明 SS-hAFSCs 和 RS-hAFSCs 在神经保护能力上存在差异，表明 P7 缺氧缺血小鼠对侧单次注射 SS-hAFSCs 可使海马组织损失减少 47%，海马和纹状体中 TUNEL 阳性细胞减少 43-45%，48 小时后损伤，减少小胶质细胞活化和 TGFβ1 水平，并防止脱髓鞘。另一方面，RS-hAFSCs 未能显示出这种神经保护作用。SS-hAFSCs 可能通过靶细胞中内格兰蛋白依赖性 TGFβ1 信号抑制发挥其神经保护作用。这些发现确定了 CD117CD90CD105 干细胞的一个亚群作为发育中大脑神经保护的有希望的来源。
