Muchová Katarína, Chromiková Zuzana, Valenčíková Romana, Barák Imrich
Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia.
Front Microbiol. 2018 Jan 18;8:2650. doi: 10.3389/fmicb.2017.02650. eCollection 2017.
Vegetative cell division in takes place precisely at the middle of the cell to ensure that two viable daughter cells are formed. The first event in cell division is the positioning of the FtsZ Z-ring at the correct site. This is controlled by the coordinated action of both negative and positive regulators. The existence of positive regulators has been inferred, but none have presently been identified in . Noc and the Min system belong to negative regulators; Noc prevents division from occurring over the chromosomes, and the Min system inhibits cell division at the poles. Here we report that the morphogenic protein, RodZ, an essential cell shape determinant, is also required for proper septum positioning during vegetative growth. In mutant cells, the vegetative septum is positioned off center, giving rise to small, round, DNA-containing cells. Searching for the molecular mechanism giving rise to this phenotype led us to discover that RodZ directly interacts with MinJ. We hypothesize that RodZ may aid the Min system in preventing non-medial vegetative division.
营养细胞分裂精确发生在细胞中部，以确保形成两个有活力的子细胞。细胞分裂的第一个事件是FtsZ Z环在正确位置的定位。这由负调控因子和正调控因子的协同作用控制。正调控因子的存在已被推断，但目前在[具体物种]中尚未鉴定出任何正调控因子。Noc和Min系统属于负调控因子；Noc可防止在染色体上发生分裂，而Min系统抑制两极的细胞分裂。在此，我们报道形态发生蛋白RodZ（一种必需的细胞形状决定因子）在营养生长期间对于隔膜的正确定位也是必需的。在[具体物种]突变细胞中，营养隔膜偏离中心定位，产生小的、圆形的、含DNA的细胞。对导致这种表型的分子机制的研究使我们发现RodZ直接与MinJ相互作用。我们推测RodZ可能协助Min系统防止非中间位置的营养细胞分裂。
