Guo Jia, Islam Md Ashraful, Lin Haocheng, Ji Changan, Duan Yinghui, Liu Peng, Zeng Qingdong, Day Brad, Kang Zhensheng, Guo Jun
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, China.
Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, United States.
Front Plant Sci. 2018 Jan 22;9:18. doi: 10.3389/fpls.2018.00018. eCollection 2018.
Cyclic nucleotide gated channels (CNGCs) play multifaceted roles in plants, particularly with respect to signaling processes associated with abiotic stress signaling and during host-pathogen interactions. Despite key roles during plant survival and response to environment, little is known about the activity and function of CNGC family in common wheat ( L.), a key stable food around the globe. In this study, we performed a genome-wide identification of CNGC family in wheat and identified a total 47 in wheat, classifying these genes into four major groups (I-IV) with two sub-groups (IVa and IVb). Sequence analysis revealed the presence of several conserved motifs, including a phosphate binding cassette (PBC) and a "hinge" region, both of which have been hypothesized to be critical for the function of wheat CNGCs. During wheat infection with , the transcript levels of and , both members of group IVb, showed significant induction during a compatible interaction, while a reduction in gene expression was observed in incompatible interactions. In addition, and mRNA accumulation was significantly influenced by exogenously applied hormones, including abscisic acid (ABA), methyl jasmonate (MeJA), and salicylic acid (SA), suggesting a role in hormone signaling and/or perception. Silencing of and limited growth and increased wheat resistance against . The results presented herein contribute to our understanding of the wheat CNGC gene family and the mechanism of signaling during wheat- interaction.
环核苷酸门控通道（CNGCs）在植物中发挥着多方面的作用，特别是在与非生物胁迫信号传导相关的信号传导过程以及宿主 - 病原体相互作用期间。尽管在植物生存和对环境的响应中起关键作用，但对于全球重要的稳定粮食作物普通小麦（Triticum aestivum L.）中CNGC家族的活性和功能却知之甚少。在本研究中，我们对小麦中的CNGC家族进行了全基因组鉴定，共鉴定出47个小麦CNGC基因，将这些基因分为四个主要组（I - IV）和两个亚组（IVa和IVb）。序列分析揭示了几个保守基序的存在，包括一个磷酸盐结合盒（PBC）和一个“铰链”区域，据推测这两个区域对于小麦CNGCs的功能至关重要。在小麦感染条锈菌（Puccinia striiformis f. sp. tritici）期间，IVb组的两个成员TaCNGC13和TaCNGC14的转录水平在亲和互作过程中表现出显著诱导，而在非亲和互作中观察到基因表达降低。此外，TaCNGC13和TaCNGC14 mRNA的积累受到外源施加的激素的显著影响，包括脱落酸（ABA）、茉莉酸甲酯（MeJA）和水杨酸（SA），表明它们在激素信号传导和/或感知中发挥作用。TaCNGC13和TaCNGC TaCNGC14的沉默限制了条锈菌的生长并增强了小麦对条锈菌的抗性。本文呈现的结果有助于我们理解小麦CNGC基因家族以及小麦与条锈菌互作期间的信号传导机制。
