, a Yellow Stripe-Like Transporter Gene, Is Required for Wheat Resistance to f. sp. .

Yellow stripe-like (YSL) transporters are required for the transportation of metal-phytosiderophores and are structurally related to metal-nicotianamine complexes. Some studies also reported the involvement of YSL transporters in pathogen-induced defense. However, the molecular mechanisms of genes involved in biotic stress responses are still not clear, especially in cereal crops. This study aimed to functionally characterize during the interaction of wheat and f. sp. (), the causal agent of stripe rust disease. TaYS1A was localized in the cell membrane of wheat protoplasts and cells. was significantly up-regulated in wheat leaves after being infected with the avirulent isolate CYR23 and after treatment with salicylic acid (SA). Silencing of by the virus-induced gene silencing method enhanced the susceptibility of wheat to accompanied by reducing the accumulation of SA and HO and down-regulating the transcriptions of and . In addition, TaYS1A was found to interact with TaNH2, a homolog of OsNH2, by yeast-two-hybrid assay, and silencing of diminished the expression of . Our findings suggested the existence of positive regulation of in providing resistance against by modulating SA-induced signaling and offered new insight into the biological role of in wheat against pathogens.