Ariede Raquel B, Freitas Milena V, Hata Milene E, Mastrochirico-Filho Vito A, Pilarski Fabiana, Batlouni Sergio R, Porto-Foresti Fábio, Hashimoto Diogo T
Aquaculture Center of Unesp, São Paulo State University (Unesp), Jaboticabal, Brazil.
School of Sciences, São Paulo State University (Unesp), Bauru, Brazil.
Front Genet. 2018 Jan 18;9:3. doi: 10.3389/fgene.2018.00003. eCollection 2018.
Tambaqui, , is the main native fish species produced in Brazil, and is an important species for genetic improvement in aquaculture. In addition, breeding studies on this species can be optimized with the use of molecular markers associated with productive phenotypes. The objective of the present study was to test the performance of growth traits and resistance to the bacteria, , in association with microsatellite markers in . In this study, three full-sib families were subjected to bacterial challenge and morphometric growth assessments. Tambaqui families subjected to the bacterial challenge differed significantly in death time and mortality rate. There was, however, no association between resistance to bacteria and microsatellite markers. In relation to growth traits, we observed a marker/phenotype association in two microsatellites. The marker in the 6b isoform x5 gene () was associated with length, whereas an anonymous marker was associated with height. The present study highlighted the evaluation of molecular markers associated with growth traits, and can serve as the basis for future marker-assisted selection (MAS) of tambaqui.
淡水白鲳是巴西主要的本土养殖鱼类品种,也是水产养殖遗传改良的重要品种。此外,利用与生产性状相关的分子标记可优化该品种的育种研究。本研究的目的是测试淡水白鲳生长性状的表现以及对嗜水气单胞菌的抗性,并将其与微卫星标记相关联。在本研究中,三个全同胞家系接受了细菌攻毒试验和形态生长评估。接受细菌攻毒试验的淡水白鲳家系在死亡时间和死亡率上存在显著差异。然而,对细菌的抗性与微卫星标记之间没有关联。关于生长性状,我们在两个微卫星中观察到标记/表型关联。6b 同工型 x5 基因()中的标记与体长相关,而一个匿名标记与体高相关。本研究突出了对与生长性状相关的分子标记的评估,可为未来淡水白鲳的标记辅助选择(MAS)奠定基础。