Frickmann Hagen, Hahn Andreas, Schwarz Norbert Georg, Hagen Ralf Matthias, Dekker Denise, Hinz Rebecca, Micheel Volker, Hogan Benedikt, May Jürgen, Rakotozandrindrainy Raphael
Department of Tropical Medicine at the Bernhard Nocht Institute, Bundeswehr Hospital Hamburg, Hamburg, Germany.
Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, Rostock, Germany.
Eur J Microbiol Immunol (Bp). 2017 Oct 19;7(4):274-277. doi: 10.1556/1886.2017.00028. eCollection 2017 Dec 18.
Direct growth on blood and screening agar for methicillin-resistant (MRSA) at a tropical surveillance site was compared with broth enrichment and subsequent growth on selective MRSA agar after international sample transport. In Madagascar, 1548 swabs from an MRSA surveillance study were assessed for growth on Columbia blood agar enriched with 5% sheep blood and MRSA screening agar at the surveillance site with subsequent cold storage of the samples and shipment to Germany. In Germany, 1541 shipped samples were analyzed by non-selective broth enrichment with subsequent culture on MRSA selective agar. A total of 28 MRSA isolates were detected. Of these, 20 strains were isolated from direct culture on blood and MRSA screening agars at the surveillance site, 24 MRSA strains were isolated using the broth enrichment method in Germany, and 16 MRSA strains were identified by both approaches. In spite of the observed die-off of individual strains due to long-term storage and transport, broth enrichment with subsequent screening on MRSA selective agar after international sample shipment led to comparable sensitivity of MRSA detection like streaking on blood and MRSA agar at the tropical surveillance site.
在一个热带监测点，将耐甲氧西林金黄色葡萄球菌（MRSA）在血琼脂和筛选琼脂上的直接培养，与国际样本运输后肉汤增菌并随后在选择性MRSA琼脂上培养的情况进行了比较。在马达加斯加，对一项MRSA监测研究中的1548份拭子样本，在监测点评估其在含5%羊血的哥伦比亚血琼脂和MRSA筛选琼脂上的生长情况，随后对样本进行冷藏并运往德国。在德国，对1541份运送来的样本进行非选择性肉汤增菌分析，随后在MRSA选择性琼脂上培养。共检测到28株MRSA分离株。其中，20株菌株是在监测点通过血琼脂和MRSA筛选琼脂上的直接培养分离得到的，24株MRSA菌株是在德国采用肉汤增菌法分离得到的，16株MRSA菌株通过两种方法均被鉴定出来。尽管观察到由于长期储存和运输个别菌株死亡，但国际样本运输后肉汤增菌并随后在MRSA选择性琼脂上筛选，导致MRSA检测的敏感性与在热带监测点血琼脂和MRSA琼脂上划线培养相当。
