微波辅助萃取用于高效液相色谱-荧光法测定[具体物质]中葛根素和大豆苷元的响应面优化

Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in .

作者信息

Liu Ying-Kun, Yan E, Zhan Han-Ying, Zhang Zhi-Qi

机构信息

Key Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry (Shaanxi Normal University), Ministry of Education, Xi'an 710062, China.

Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Forestry University, Lin'an 311300, China.

出版信息

J Pharm Anal. 2011 Feb;1(1):13-19. doi: 10.1016/S2095-1779(11)70003-X. Epub 2012 Jan 30.

Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in The optimized extraction procedure was achieved by soaking the sample with 70% methanol (1:15, v/v) for 30 min, and then microwave irradiation for 11 min at a power of 600 W. Coupling the extraction process with HPLC-fluorescence presented good recovery, satisfactory precision, and good linear relation. Compared with a method from the Chinese Pharmacopoeia, the proposed method enables higher extraction efficiency and more aecurate analytical results. It can be of Potential value in quality assessment of medicinal materials.