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微波辅助萃取用于高效液相色谱-荧光法测定[具体物质]中葛根素和大豆苷元的响应面优化

Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in .

作者信息

Liu Ying-Kun, Yan E, Zhan Han-Ying, Zhang Zhi-Qi

机构信息

Key Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry (Shaanxi Normal University), Ministry of Education, Xi'an 710062, China.

Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Forestry University, Lin'an 311300, China.

出版信息

J Pharm Anal. 2011 Feb;1(1):13-19. doi: 10.1016/S2095-1779(11)70003-X. Epub 2012 Jan 30.

DOI:10.1016/S2095-1779(11)70003-X
PMID:29403676
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5760777/
Abstract

Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in The optimized extraction procedure was achieved by soaking the sample with 70% methanol (1:15, v/v) for 30 min, and then microwave irradiation for 11 min at a power of 600 W. Coupling the extraction process with HPLC-fluorescence presented good recovery, satisfactory precision, and good linear relation. Compared with a method from the Chinese Pharmacopoeia, the proposed method enables higher extraction efficiency and more aecurate analytical results. It can be of Potential value in quality assessment of medicinal materials.

摘要

采用响应面法对微波辅助萃取进行优化，用于高效液相色谱-荧光法测定葛根中葛根素和大豆苷元。优化后的萃取程序为：将样品用70%甲醇（1:15，v/v）浸泡30分钟，然后在600瓦功率下微波辐射11分钟。将萃取过程与高效液相色谱-荧光法联用，回收率良好，精密度令人满意，线性关系良好。与《中国药典》中的方法相比，该方法具有更高的萃取效率和更准确的分析结果。它在药材质量评价中具有潜在价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e786/5760777/b732ce4586d5/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e786/5760777/9672fc9d0770/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e786/5760777/98e708b3e9f8/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e786/5760777/2c017ae8ca3b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e786/5760777/aed993e8e48c/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e786/5760777/b732ce4586d5/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e786/5760777/9672fc9d0770/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e786/5760777/98e708b3e9f8/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e786/5760777/2c017ae8ca3b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e786/5760777/aed993e8e48c/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e786/5760777/b732ce4586d5/gr5.jpg

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