Zhao Hong-Xia, Mu Hui, Bai Yan-Hong, Yu Hu, Hu Ying-Mei
Chemistry Department of Science School, Xi'an Jiaotong University, Xi'an 710049, China.
J Pharm Anal. 2011 Aug;1(3):208-212. doi: 10.1016/j.jpha.2011.04.003. Epub 2011 Jul 22.
A rapid method has been developed based on the sample preparation procedure named as QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe), combined with reversed-phase high performance liquid chromatography with fluorescence detector and C column after pre-column derivatization using -phthalaldehyde and 2-mercaptoethanol to determine dopamine in porcine muscle. Methanol and deionized water (0.1% acetic acid, v/v) with a ratio of 60:40 was used as mobile phase. The flow rate was 0.8 mL/min and dopamine was eluted within 15 min. The linearity range was 0.003-8 μg/mL with =0.9992. The detection limit for dopamine was 4 μg/kg and the quantification limit was 9 μg/kg. Recovery studies were carried out at 0.1, 0.5 and 1.0 mg/kg fortification levels and the average recoveries obtained ranged from 90.4% to 98.2% with relative standard deviations between 3.5% and 8.1%. The method was found to be suitable for detection of dopamine in animal product tissues at the maximum residue level.
基于名为QuEChERS（快速、简便、廉价、有效、耐用且安全）的样品制备程序，开发了一种快速方法，该方法结合了反相高效液相色谱法，使用荧光检测器和C柱，在使用邻苯二甲醛和2-巯基乙醇进行柱前衍生化后测定猪肌肉中的多巴胺。以体积比为60:40的甲醇和去离子水（0.1%乙酸，v/v）作为流动相。流速为0.8 mL/min，多巴胺在15分钟内洗脱。线性范围为0.003 - 8 μg/mL，相关系数为0.9992。多巴胺的检测限为4 μg/kg，定量限为9 μg/kg。在0.1、0.5和1.0 mg/kg的加标水平下进行回收率研究，获得的平均回收率在90.4%至98.2%之间，相对标准偏差在3.5%至8.1%之间。该方法被认为适用于检测动物产品组织中最大残留水平的多巴胺。
