高效液相色谱法同时测定中药“舒筋止痛”胶囊中五种主要活性成分的含量

Yang Xing-Xin, Zhang Xiao-Xia, Chang Rui-Miao, Wang Yan-Wei, Li Xiao-Ni

College of Pharmacy, Shanxi Medical University, 56 Xinjian South Road, Taiyuan 030001, China.

J Pharm Anal. 2011 Nov;1(4):284-290. doi: 10.1016/j.jpha.2011.08.002. Epub 2011 Oct 22.

A simple and reliable high performance liquid chromatography (HPLC) method has been developed for the simultaneous quantification of five major bioactive components in 'Shu-Jin-Zhi-Tong' capsules (SJZTC), for the purposes of quality control of this commonly prescribed traditional Chinese medicine. Under the optimum conditions, excellent separation was achieved, and the assay was fully validated in terms of linearity, precision, repeatability, stability and accuracy. The validated method was applied successfully to the determination of the five compounds in SJZTC samples from different production batches. The HPLC method can be used as a valid analytical method to evaluate the intrinsic quality of SJZTC.

已开发出一种简单可靠的高效液相色谱（HPLC）方法，用于同时定量测定“舒筋止痛”胶囊（SJZTC）中的五种主要生物活性成分，以对这种常用的传统中药进行质量控制。在最佳条件下，实现了出色的分离效果，并且该测定方法在线性、精密度、重复性、稳定性和准确性方面均得到了充分验证。经过验证的方法成功应用于不同生产批次SJZTC样品中这五种化合物的测定。该HPLC方法可作为评估SJZTC内在质量的有效分析方法。