Qiao Chun-Feng, Han Quan-Bin, Song Jing-Zheng, Mo Shi-Fu, Kong Ling-Dong, Kung Hsiang-Fu, Xu Hong-Xi
Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Shatin, Hong Kong, China.
J Sep Sci. 2007 Apr;30(6):813-8. doi: 10.1002/jssc.200600339.
Fructus Psoraleae, a widely used traditional Chinese medicine, is well known as a health supplement ingredient. In our study, an improved and comprehensive HPLC fingerprint of Fructus Psoraleae was established. Two important new benzofuran glycosides, psoralenoside and isopsoralenoside, were identified as characteristic constituents for the first time. HPLC separation was performed on an RP-C8 column. The mobile phase was acetonitrile and 0.1% acetic acid solution with linear gradient change of acetonitrile from 10 to 82% in 40 min. The flow rate was 1.0 mL/min, and the detection wavelength was set at 310 nm. The HPLC chromatograms of twenty-six samples from different regions of China showed a similar pattern. Twelve peaks were selected as characteristic peaks and further identified as psoralenoside, isopsoralenoside, psoralen, isopsoralen, bavachromene, corylifolin, corylin, psoralidin, isobavachalcone, bavachinin, corylifol A, and bakuchiol, respectively. Nine of them were simultaneously quantitatively analyzed for the first time. A more comprehensive analytical method was established for the fingerprint of Fructus Psoraleae. It is very useful for authentication and quality assessment of the crude drug.
补骨脂，一种广泛使用的传统中药，作为一种健康补充剂成分而广为人知。在我们的研究中，建立了一种改进的、全面的补骨脂高效液相色谱指纹图谱。首次鉴定出两种重要的新苯并呋喃糖苷，补骨脂苷和异补骨脂苷，作为特征成分。在RP-C8柱上进行高效液相色谱分离。流动相为乙腈和0.1%乙酸溶液，乙腈在40分钟内从10%线性梯度变化至82%。流速为1.0 mL/min，检测波长设定为310 nm。来自中国不同地区的26个样品的高效液相色谱图显示出相似的模式。选择12个峰作为特征峰，并进一步分别鉴定为补骨脂苷、异补骨脂苷、补骨脂素、异补骨脂素、补骨脂色烯、补骨脂次素、补骨脂宁、补骨脂定、异补骨脂查尔酮、补骨脂宁、补骨脂甲素和补骨脂酚。其中9种首次同时进行了定量分析。建立了一种更全面的补骨脂指纹图谱分析方法。它对该药材的鉴定和质量评价非常有用。
