Li Ya, Fu Qiang, Liu Meng, Jiao Yuan-Yuan, Du Wei, Yu Chong, Liu Jing, Chang Chun, Lu Jian
Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, PR China.
Department of Pharmacy, Northwest Hospital, Xi'an 710004, PR China.
J Pharm Anal. 2012 Dec;2(6):395-402. doi: 10.1016/j.jpha.2012.08.002. Epub 2012 Sep 11.
In order to prepare a high capacity packing material for solid-phase extraction with specific recognition ability of trace ractopamine in biological samples, uniformly-sized, molecularly imprinted polymers (MIPs) were prepared by a multi-step swelling and polymerization method using methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, and toluene as a porogen respectively. Scanning electron microscope and specific surface area were employed to identify the characteristics of MIPs. Ultraviolet spectroscopy, Fourier transform infrared spectroscopy, Scatchard analysis and kinetic study were performed to interpret the specific recognition ability and the binding process of MIPs. The results showed that, compared with other reports, MIPs synthetized in this study showed high adsorption capacity besides specific recognition ability. The adsorption capacity of MIPs was 0.063 mmol/g at 1 mmol/L ractopamine concentration with the distribution coefficient 1.70. The resulting MIPs could be used as solid-phase extraction materials for separation and enrichment of trace ractopamine in biological samples.
为制备具有对生物样品中痕量莱克多巴胺特异性识别能力的用于固相萃取的高容量填充材料，分别以甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂、甲苯为致孔剂，采用多步溶胀聚合法制备了粒径均匀的分子印迹聚合物（MIPs）。利用扫描电子显微镜和比表面积来鉴定MIPs的特性。通过紫外光谱、傅里叶变换红外光谱、Scatchard分析和动力学研究来解释MIPs的特异性识别能力和结合过程。结果表明，与其他报道相比，本研究合成的MIPs除具有特异性识别能力外，还表现出高吸附容量。在莱克多巴胺浓度为1 mmol/L时，MIPs的吸附容量为0.063 mmol/g，分配系数为1.70。所得MIPs可作为固相萃取材料用于生物样品中痕量莱克多巴胺的分离和富集。
